BIS 103: gluconeogenesis

Question 1

allosteric enzymes

Answer 1

pyruvate carboxylase, PEP carboxykinase,

fructose-1,6-biphosphatase, glucose-6-phosphatase

Question 2

enzymes that add E to avoid PEP

Answer 2

pyruvate carboxylase , ATP

PEP carboxykinase, GTP

Question 3

where does pyruvate carboxylase work?

Answer 3

mitochondrial matrix

Question 4

where does PEP carboxykinase work?

Answer 4

cytosol

Question 5

malate dehygrogenase

Answer 5

2 isozymes converts oaa so can go from matrix to cytosol and back,
uses 2 pools of NADH as needed

Question 6

which iD enzyme is not allosteric

Answer 6

g6Phosphatase

Question 7

Gluconeogenesis: ATP /1 glucose?

enzymes

Answer 7

4 - pyruvate carboxylase, phosphoglycerokinase

Question 8

Gluconeogenesis: GTP/1 glucose?

enzymes

Answer 8

2 -PEP carboxykinase

Question 9

Gluconeogenesis: NADH/1 glucose?

enzymes

Answer 9

2 - g3p dehydrogenase

Question 10

Gluconeogenesis: waters /1 glucose?

enzymes

Answer 10

4 - fructose-1,6-biphosphatase, glucose-6-phosphatase

Question 11

what tether is used in gluconeo?

Answer 11

biotinyl-Lys

in pyruvate carboxylase

Question 12

mech for PDH-like

Answer 12

pyruvate carboxylase-
E1: co2 binds to biotinyl-lys
bring to E2: add Co2 to pyruvate -> oxalo