BIS 103: Photosynthesis III

Question 1

3 stages of Calvin cycle

Answer 1

fixation

, reduction, regeneration of acceptor

Question 2

first stage of Calvin cycle

Answer 2

CO2 + 5C sugar ribulose - 1,5- phosphate–>2 phosphoglycerate

1C + 5C –> 2 X 3C

Question 3

second stage of Calvin

Answer 3

use ATP(2/1C) and NADPH (2/C) to make aldehyde from CO2

make glyceraldehyde 3 phosphate

Question 4

stage 3 of Calvin cycle

Answer 4

use ATP (1/C) to regenerate rilose - 1,5- biphosphate

Question 5

Enzyme of 1st stage of Calvin

Answer 5

Rubisco

Question 6

small rate of rubisco –>

Answer 6

most abundant

Question 7

active site of rubisco

Answer 7

Mg ion cofactor
CO2 is a coenzyme
(CO2-Lys)

Question 8

What enzyme uses NADPH?

Answer 8

glyceraldehyde - 3 -phosphate dehydrogenase - isozyme - reverse of glycolysis

Question 9

What isozyme from glycolysis is missing from Calvin?

Answer 9

phosphyglucomutase

Question 10

how is P conserved within chloroplast?

Answer 10

antiport - sugar-P, P

Question 11

how many protons needed to make 1 atp?

Answer 11

4

Question 12

what path is stage 3?
significant enzymes?
sig substrates

Answer 12

c shuffle by reductive pentose phosphate pathway
transketolase, aldolase

ATP - 1/C

Question 13

1-glyceraldehyde3P: use

Answer 13

3CO2, 1H2O, 9ATP, 6NADPH