Calculations Of Blood Components

Question 1

refers to the process of separating donated whole blood into its components—red blood cells (RBCs), platelets, plasma, cryoprecipitate—so that each part can be transfused as needed to different patients.

Answer 1

Blood Component Therapy

Question 2

Mathematical Calculations in Blood Transfusion

🧾 Goals:

Answer 2

1. Determine the required amount of blood component to be transfused
   
   2. Estimate the expected therapeutic effect

Question 3

Hemoglobin-Based Calculation

💡 Key Principle:
• 1 unit of RBC (whole blood or PRBC) increases hemoglobin by approximately ______in a 70 kg adult.

Answer 3

1 g/dL

Question 4

CASE 1: Hemoglobin Correction

📄 Case Information:
• Patient X’s current Hb: 7.8 g/dL
• Target Hb: 12.5 g/dL

Answer 4

4.7 = 5 units

Question 5

Hematocrit-Based Calculation

💡 Key Principle:
• 1 unit of PRBC increases hematocrit by____ in a 70 kg adult.

Answer 5

~3%

Question 6

CASE 2: Hematocrit Correction

📄 Case Information:
• Patient Y’s current Hct: 20%
• Target Hct: 32%

Answer 6

12/3 =4 units

Question 7

Hematocrit-Based Calculation

💡 Key Principle:
• 1 unit of PRBC increases hematocrit by____ in a 70 kg adult.

Answer 7

~3%

Question 8

is a commercial preparation of human anti-D IgG antibodies used for immune prophylaxis in Rh-negative (Rh–) individuals who may be exposed to Rh-positive (Rh⁺) red blood cells.

Answer 8

RhoGAM

Question 9

RhoGAM is a commercial preparation of human anti-D IgG antibodies used for immune prophylaxis in _______individuals who may be exposed to_______ red blood cells.

Answer 9

Rh-negative (Rh–)

Rh-positive (Rh⁺)

Question 10

RhoGAM

Given within:
______ after delivery, abortion, trauma, or invasive procedures (like amniocentesis) if the baby is Rh⁺ and the mother is Rh⁻.

• 🧊 Storage temperature:_____
• 📦 Shelf life:_____

Answer 10

72 hours

1°C to 6°C

2 years

Question 11

RhoGAM

• Full dose (standard): _______
• Mini dose:_______ (used for early pregnancies or small fetomaternal hemorrhages)

Answer 11

300 µg

120 µg or 50 µg

Question 12

A single vial of Rhig is sufficient to compensate:
_______red cells (packed red blood cell)
_______whole blood/fetomaternal bleed

Answer 12

15 mL D(+)

30 mL D(+)

Question 13

Volume FMH (mL):

Dose of Rhig:

Answer 13

% × 50

Volume of FMH/30

Question 14

Case Summary:
• Mother’s blood type: Rh⁻
• Baby’s blood type: Rh⁺
• Timing: 72 hours postpartum
• Test done: Kleihauer-Betke
• Result: 3.2% fetal cells in maternal blood
• Dose required: Full dose (300 µg)

Answer 14

3.2 x 50 =160
160/ 30 = 5.333 + 1= 6 vials

Question 15

RhoGAM

Note:
1. Follow the rule in rounding off a decimal number to a whole number. ADD ______to the final answer.
2. Fifty (50) is a factor derived by dividing 5,000 (average mother’s blood volume) by 100. Thus, to calculate volume FMH, drop off % sign and multiply the number by____.
3. Rhig may also be used for the treatment of _______aside from the prevention of Rh HDN. 300 ug (full dose) and 120 ug are two FDA-approved doses.

Answer 15

1 VIAL

50

idiopathic thrombocytopenic purpura (ITP)

Question 16

are small, anucleate cell fragments derived from megakaryocytes.

• They play a crucial role in_______, forming the initial platelet plug at sites of vascular injury.

Answer 16

Platelets (Thrombocytes)

primary hemostasis

Question 17

Platelet Transfusions are Indicated for:

Thrombocytopenia (low platelet count) due to:

Answer 17

🔸 Defective production (e.g., aplastic anemia, leukemia, chemotherapy)

🔸 Increased destruction (e.g., immune thrombocytopenia, dengue)

🔸 Decreased function (e.g., uremia, antiplatelet drugs)

Question 18

Platelet Count

RDP
SDP

Answer 18

5.5 × 10¹⁰
3 × 10¹¹

Question 19

Platelets

Shelf life:_______ (If pooled platelets, within______ of pooling)

pH:______

Answer 19

3-5 days

4 hours

> 6.2

Question 20

Platelets
• Each unit of platelet is expected to provide the ff. increase in platelet count (70 kg human)
RDP:________
SDP:________ (same effect with 4-6 pools of RDP)

Answer 20

5,000 -10,000/ul

20,000-60,000/ul

Question 21

Purpose:
• To evaluate the effectiveness of a platelet transfusion.
• A low ____ suggests immune refractoriness, poor quality product, or ongoing consumption/bleeding.

Answer 21

CORRECTED COUNT INCREMENT (CCI)

Question 22

• To assess the efficiency of platelet extraction during component preparation.

• It reflects how much of the original platelet content from whole blood was retained in the platelet-rich plasma (PRP) or platelet concentrate (PC).

Answer 22

PLATELET RECOVERY CALCULATION

Question 22

CCI RDP=

CCI SDP=

where PC = platelet count
BSA = body surface in square meter

Answer 22

(Post-transfusion PC- Pre-transfusion PC) x BSA/
number of RDP transfused × 0.55

Post-transfusion PC - Pre-transfusion PC) × BSA/
number of SDP transfused × 3

Question 23

is the cold-insoluble portion of plasma that precipitates when_____ is thawed slowly at 1–6 °C.

The precipitate is then centrifuged and collected, and the supernatant is removed. This concentrates certain plasma proteins.

Answer 23

Cryoprecipitate, also called Cryo,

fresh frozen plasma (FFP)

Question 24

Composition & Therapeutic Uses Cryoprecipitate contains high concentrations of:

Answer 24

Fibrinogen Fibronectin FVIII FXIII

Question 25

Cryoprecipitate Storage: Frozen

Answer 25

-18 °C or lower 1 year

Question 26

Cryoprecipitate Storage: Thawed (unpooled) Pooled (open system)

Answer 26

20–24 °C 6 hours 4 hours

Question 27

Cryoprecipitate Quality Control (per 1 unit): • Fibrinogen: • Factor VIII: • vWF: • Factor XIII:

Answer 27

150–250 mg 80–120 IU 40–70% 20–30%