Gel Technology

Question 1

GEL TECHNOLOGY
• Based on_______

• Developed by_______

Answer 1

Column agglutination technology (CAT)

Dr. Yves Lapierre (Lyon, France)

Question 2

Gel Tech

Modern crossmatching technique that uses microtubes filled with gel particles to detect agglutination between:

Answer 2

• Donor red blood cells (RBCs) 💉
• Recipient serum or plasma 🧪

Question 3

• A modern method of crossmatching that uses a *microtube filled with gel particles to detect agglutination between donor red blood cells and recipient serum or plasma.

Answer 3

Gel Technology

Question 4

• 💥 _______reactions occur when the recipient’s_____ recognize and bind to antigens on the donor’s_____.

• 🧬 The gel acts like a molecular sieve:
• It allows free (non-agglutinated) red cells to pass through to the bottom of the microtube.
• It traps large agglutinates (clumped RBCs bound by antibodies) at the top or within the gel matrix.

Answer 4

Red cell-antibody

antibodies

RBCs

Question 5

Helps to eliminate subjectivity in result interpretation and increase accuracy.

Answer 5

DG Reader

Question 5

is an automated result interpretation device used in gel column agglutination technology (CAT).

Answer 5

DG Reader

Question 6

GEL TECHNOLOGY
DISADVANTAGES

Answer 6

Cost
False Positives
Limited Sensitivity for IgM antibodies
Need for special equipment

Question 7

GEL TECHNOLOGY
ADVANTAGES

Answer 7

High Sensitivity and Specificity
Standardization
Stable Endpoint
Reduced Technical Errors
Automation Ready