Cell Fractionation

Question 1

What is the process of cell fractionation?

Answer 1

Cells are broken up and the different organelles they contain are separated out

Question 2

What are the 2 stages of cell fractionation?

Answer 2

Homogenation

Ultracentrifugation

Question 3

What are cells placed in?

Answer 3

A buffered solution

Question 4

What must the buffered solution be?

Answer 4

Ice cold
Buffered (same pH)
Isotonic

Question 5

WhAt happens in homogenation

Answer 5

Disrupting the tissue and cells

Question 6

What happens during ultracentrifugation?

Answer 6

Separating the organelle in a centrifuge

Question 7

How can you predict where the organelle will be in?

Answer 7

Depending on the density of the organelle

Question 8

Homogenation stages

Answer 8

Break cells with high frequency sound
Use a mild detergent to make holes in the plasma membrane
Force cells through a small hole using high pressure
Shear cells between a close fitting rotating plunger and the thick walls of glass vessel