Flashcards in CellSig2 - 15 Deck (15):
5 ways in which receptor-ligand binding occurs
Ionic attraction, van der Waals forces, hydrogen bonding, hydrophobic interaction, surface topology
3 excitatory NTs
ACh, Glu, Serotonin
2 inhibitory NTs
What are the three states of transmitter gated ion channels?
Unoccupied (closed), occupied (open), occupied (closed)(inactivated)
Characterise standard AChR
2 alpha, beta, delta, gamma
Where does ACh bind to AChRs?
2x on gamma
Five classes of enzyme-linked receptors
Receptor tyrosine kinases, tyrosine-kinase associated receptors, receptor-like tyrosine phosphatases, receptor serine/threonin kinases, receptor guanylyl cyclases
Outline a tyrosine-kinase linked receptor pathway
Alpha-interferon binds receptors and JAKs cross phosphorylate each other, then their adjacent receptor tyrosines, activating STATs
Outline a receptor threonine/serine kinase pathway
TGFb binds types 1 and 2 TGFb receptors (heterodimer), type 2 > p-type 1 > Smad2 or 3 recruitment > pSmad2 or 3
Characterise receptor guanylyl cyclase
1TM with EC ligand binding site
What is the function of guanylyl cyclase?
IC domain catalyses cGMP formation
Outline the guanylyl cyclase pathway
Ligand binds, cGMP forms, then binds and activates cGMP-dependent protein kinase or ion channels
What are the signal molecules for guanylyl cyclase?
Due to interactions between ligans and or binding sites in a receptor complex - negative results in binding of one receptor site decreasing affinity at other sites; positive is vice versa