CellSig2 - 15 Flashcards Preview

Y2S2 - Cell Signalling > CellSig2 - 15 > Flashcards

Flashcards in CellSig2 - 15 Deck (15):
1

5 ways in which receptor-ligand binding occurs

Ionic attraction, van der Waals forces, hydrogen bonding, hydrophobic interaction, surface topology

2

3 excitatory NTs

ACh, Glu, Serotonin

3

2 inhibitory NTs

GABA, Glycine

4

What are the three states of transmitter gated ion channels?

Unoccupied (closed), occupied (open), occupied (closed)(inactivated)

5

Characterise standard AChR

2 alpha, beta, delta, gamma

6

Where does ACh bind to AChRs?

2x on gamma

7

Five classes of enzyme-linked receptors

Receptor tyrosine kinases, tyrosine-kinase associated receptors, receptor-like tyrosine phosphatases, receptor serine/threonin kinases, receptor guanylyl cyclases

8

Outline a tyrosine-kinase linked receptor pathway

Alpha-interferon binds receptors and JAKs cross phosphorylate each other, then their adjacent receptor tyrosines, activating STATs

9

Outline a receptor threonine/serine kinase pathway

TGFb binds types 1 and 2 TGFb receptors (heterodimer), type 2 > p-type 1 > Smad2 or 3 recruitment > pSmad2 or 3

10

Characterise receptor guanylyl cyclase

1TM with EC ligand binding site

11

What is the function of guanylyl cyclase?

IC domain catalyses cGMP formation

12

Outline the guanylyl cyclase pathway

Ligand binds, cGMP forms, then binds and activates cGMP-dependent protein kinase or ion channels

13

What are the signal molecules for guanylyl cyclase?

Natriuretic peptides

14

Outline cooperativity

Due to interactions between ligans and or binding sites in a receptor complex - negative results in binding of one receptor site decreasing affinity at other sites; positive is vice versa

15

What is the outcome of cooperativity?

Sharpening of response