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Flashcards in Cellular Architectue + Muscle Types Deck (23)
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1

What H and E stain for?

Nucleic acid and protein

2

Difference between preparation for L.M and E.M

LM - embed in wax + glass slide
EM - embed in plastic + copper grid

3

Connective tissue types

Adipose, bone, fibrous (tendon), loose (under skin)

4

Types of collagen

1. Dense irregular connective tissue (random bundle)
2. A ligament (right angles)
3. A tendon (cable)

5

Collagen polymerization occurs _____ of cell

Outside

6

Characteristics of epithelia

Linked tgt by junctions, little ECM, no blood vessels/ nerves

7

Differences in appearance between smooth, skeletal and cardiac muscle

Smooth- thin, many nuclei , non-striated
Skeletal- striated
Cardiac - striated -> special junctions + some binuclear cells

8

Skeletal muscle dimensions

length 1mm-30cm, diameter 100 microns

9

How do muscle fibres connect in skeletal muscle?

No cell to cell contact -> mechanical connection by associated connective tissue

10

S.R. in skeletal muscle

triad : 2 terminal cisternae + 1 T-tubule

11

Arrangement in S.R. of skeletal muscle

Fibres: many peripherally located nuclei + mitochondria
Contractile proteins: arrange in bundles

12

In skeletal muscle actin is anchored to

Z-line at 1 end

13

Smooth muscle dimensions

length - 0.2mm (10-600 microns), diameter 2-10 microns

14

Shape of smooth muscle

Spindle

15

How do muscle fibres connect in smooth muscle?

Electrically coupled by gap junctions -> little connective tissue

16

Nucleus + mitochondria in smooth muscle

Mononucleate - central location, few mitochondria compared to skeletal muscle

17

S.R. in smooth muscle

Poorly developed -> automatic innervation with plasmalemmal structures and no T-tubule system

18

Cardiac muscle dimensions

length 50-100 microns, diameter 10-20 microns

19

How are fibres connected in cardiac muscle?

Electrically coupled by specialed junctions

20

Features of cardiac muscle

1. Intercalated discs: desosomes + gap junctions
2. can be branched
3. can be binucleate - centrally located

21

S.R. in cardiac muscle

Lacks terminal cisternae but has T-tubule system - no defined triads

22

Contractile proteins in cardiac muscle

Arranged in branching myofibrilar network through cytoplasm

23

Difference in innervation between muscle types

1. Skeletal - M.N. axons end as motor endplates on sarcolemma
2. Smooth - Autonomic nerves
3. Controlled by specialised cardiac muscle cells within nodes e.g. Purkinje cells