ch 8

Question 1

what is complementation?

Answer 1

A recessive mutation in different genes mans that they complement each other and make the WT
- when mutations do not complement each other they are on the same gene

Question 2

how can you interpret complementation tables?

Answer 2

Identifying complementation groups
- no complementation
+ complementation
Looking horizontally to see where they match up (- at the same spot = same group) (- at different spots in different groups)

Question 3

in the bacteriophage T4 experiment what did k (lambda) and B strain tell you ?

Answer 3

-WT (not in the same group) can infect B strain and K (lambda)
-mutation can only affect B strain1

Question 4

How can you get complementation with only one copy?

Answer 4

Dual infection: simultantiously infect bacteria with 2 different bacteriophages and both chromosomes are injected into E. coli and recombination can occur (the b strain infects the rll- and rll+)

Question 5

how can you check to see if mutations are on the same gene?

Answer 5

check the mutation by complementation testing

Question 6

what can K(lambda) tell you ?

Answer 6

-K has no recombination so it can tell you if the phages are on the same gene by complementation
-There on the same gene when lysis doesn’t happen (no complementation)
-Different genes when no lysis (complementation) different genes

Question 7

what can B strain tell you?

Answer 7

-B strain tells if they are the same mutation or different since recombination can happen
-Plaques form =different mutations making recombination possible
-No plaques= same mutation making it difficult for recombination

Question 8

When infecting B strain is this a genetic screen or selection?

Answer 8

Screen because both are infected and successfully liced

Question 9

When infecting K strain is this genetic screen or selection?

Answer 9

Selection because only WT could be successfully liced

Question 10

Benzers conclusions

Answer 10

A gene constsits of different parts that can each mutate (nucleotides)
Recombination can occur between different mutable sites in the same gene
Mutations only part of a gene can prevent teh gene from functioing normally

Question 11

how can you read deletion maps to tell the order of genes?

Answer 11

(….)_______
those types of maps
you look at the column and see the missing letter and that letter goes to the number in the column