CH1 Flashcards
(55 cards)
first to efficiently observe MOs and called them animalcules
Leeuwenhoek
tiny living things Leeuwenhoek saw under the microscope at 300x
animalcules
- animalcules result from fermentation/putrefaction
- chemical changes
- to occur, needs air (vital force)
abiogenesis or spontaneous generation
- animalcules come from other animalcules causes causes fermentation/putrefaction
- must arise from other living things
biogenesis
- concluded that MOs don’t spontaneously appear
- experiment with cork: vital force
Redi
- no spontaneous generation
- MOs accumulate in in bends of flask
- let air in without MOs getting in
- experiment done by Pasteur
swan neck flask experiment
said that if there is air, then there is MOs
Tyndall
- MOs produce fermentation products, not the reverse
- heat enough to kill MOs
- pasteurization
germ theory of fermentation
- 50-60 degree C (120-140 degree F)
- kill specific unwanted MOs
- doesn’t alter taste/texture
- originally for wine
pasteurization
isolated anthrax that is harmful to cattle and pasture land
-claimed one kind of MO causes one disease
Koch
caused by rod shaped bacterium with spores and introduced controls measures
anthrax
to prove the causative agent of a disease
Koch’s postulates
Name Koch’s Postulates and modifications
- specific MOs always associate with a given disease
- MO isolated and grown in pure culture in the lab
- pure culture will produce disease
- recover the injection MO from experimentally infected organism
modifications
-viruses, other obligate parasites same diseases caused by more than one MO, one MO may cause several different diseases
- causative agent of tobacco mosaic disease and would show on leaves
- something was smaller and went through filter
- filterable
Ivanovski and Beilernck (viruses)
- first human disease known that was caused by disease
- transmitted by mosquitoes
- agent filterable
- Walter Reed
yellow fever
- prevent infection
- practiced before Koch’s Germ Theory of Disease proven
antisepsis
- used to clean hands of obstreticians from giving expecting mothers childbed fever (perpetual fever)
- Semmelweis
chlorine
Lister used this to soak his surgical dressings in to prevent antisepsis in wounds
carbolic acid (phenol)
stimulation of host to develop resistance to a specific disease
immunization
- created smallpox vaccine
- inoculated James Phipps with cowpox the with smallpox, he is resistant to smallpox
Jenner
outline Pasteur’s chicken cholera experiment
- inoculated chicken with pure culture of chicken cholera bacteria 8 week old
- after several weeks, the chicken remained healthy
- Pasteur realized he used old culture and not fresh culture
- with fresh virulent culture of chicken cholera bacteria, he inoculated the chicken previously inoculated and one that was not
- previously inoculated chicken lives and the one that wasn’t dies
drugs that treat microbial diseases
chemotherapy
introduced “magic bullet” and selective toxicity
Ehrlich
kills MOs without harming the patient
selective toxicity