Chapter 13- Molecular basis of inheritance Flashcards

(35 cards)

0
Q

Trasnformation

A

A change in genotype and phenotype due to the assimilation of external DNA

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1
Q

DNA Replication

A

when DNA is copied using RNA

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2
Q

Phages

A

Viruses that infect bacteria

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3
Q

virus

A

DNA enclosed by a protective coat if protein

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4
Q

Double Helix

A

the Shape that is defined as the shape of DNA

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5
Q

Antiparallel

A

when the subunits of DNA run in opposite directions

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6
Q

semiconservative Model

A

model of replication in which the the strands split and each of the replicate DNA have a strand of the parent

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7
Q

origins of Replicaiton

A

short stretches of DNA having a specific sequence of nucleotides, proteins find this sequence and attach to start replication

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8
Q

replication fork

A

y shaped region of DNA that are being unwound by proteins

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9
Q

helicases

A

enzymes that untwist the two parental strands and make them available as parental strands

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10
Q

single strand binding proteins

A

attach to unpaired DNA strands and keep them from unpairing

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11
Q

topoisomerase

A

goes ahead of the helicase and relieves stress in the strands

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12
Q

primer

A

initial nucleotide chain producing during DNA synthesis

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13
Q

DNA Polymerases

A

Catalyze the synthesis of new DNA by adding nucleotides to a preexisting chains

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14
Q

primase

A

enzyme that synthesizes primer

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15
Q

Okazaki Fragments

A

series of segments of lagging strand, and are synthesized discontinuously, about 1000-2000

16
Q

DNA Ligase

A

Joining the sugar-phosphate backbones of the Okazaki fragments

17
Q

Mismatch repair

A

enzymes remove and replace incorrect nucleotides

18
Q

Nuclease

A

The enzyme that cut outs the mismatched nucleotides of DNA

19
Q

Nucleotide excision repair

A

A method of replacing wrong nucleotides by using DNA Polymerase and DNA Ligase

20
Q

Telomerase

A

Catalyzes the lengthening of telomeres in eukaryotic germ cells, restoring orginal length

21
Q

Nucleiod

A

The area where DNA is stored in prokaryotic cells, not surrounded by a membrane

22
Q

Chromatin

A

The protein that DNA wraps its self around when it coils up

23
Q

Heterochromatin

A

Interphase chromatin, visible by light microscope as irregular clumps

24
Euchromatin
Less compacted and more dispersed
25
Nucleic Acid Hybridization
The base pairing of one strand of a nuclei acid to a complementary sequence of a another strand
26
Genetic Engineering
Direct manipulation of genes for practical purposes
27
Plasmid
Small circular DNA molecules that replicate separately from the bacterial chromosome
28
Recombinant DNA
When two different DNA sources are combined in vitro
29
Gene cloning
Production of multiple copies of the same gene
30
Restriction enzymes
Cut DNA at specific locations
31
Restriction Site
Point where the restriction enzyme is put in use and cuts the DNA
32
Restriction Fragment
The result of the cutting of the DNA by restriction enzymes
33
Gel electrophoresis
To see restriction fragment this technique separates by lengthy
34
Cloning Vector
A DNA molecule that can carry DNA into a host cell and replicate there