Chapter 15 Flashcards
(11 cards)
1
Q
recombinant dna molecule
A
joining vector and donor fragment
2
Q
non recombinant vector
A
vectors rejoin each other (no donor insert, dont want this)
3
Q
directional cloning
A
2 re that cut diff sequence, compatible w same nonidentical sticky ends
4
Q
in plasmids
A
origin of repl, selectable marker, lacZ (reporter), MCS, promoter
5
Q
each colony has only
A
1 type of plasmid coming from 1 molecule.
6
Q
why is cdna harder to obtain than genomic?
A
you have to know what tissue you are working with, age of sample,
7
Q
iptg
A
protein expression
8
Q
codon frequency
A
isnt universal
9
Q
complementation test are for
A
how many alleles there are in gene
10
Q
how to identify more genes that function together in genetic pathway?
A
suppressor screen
11
Q
outcome from crispr/cas 9?
A
NHEJ and homologous recomb.
