Chapter 20- Biotechnology Flashcards Preview

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Flashcards in Chapter 20- Biotechnology Deck (22):
1

DNA molecules formed when segments of DNA from two different sources

Recombinant DNA

2

The manipulation of organisms or their components to make useful products

Biotechnology

3

The direct manipulation of genes for practical purposes

Genetic engineering

4

Method if preparing well defined segments of DNA in multiple identical copies

DNA cloning

5

Small circular DNA molecules that replicate separately from bacterial chromosome

Plasmid

6

Enzyme that cut DNA molecules at a limited number of specific locations

Restriction enzyme

7

Resulting double stranded restriction fragment having at least one single stranded end

Sticky ends

8

A DNA molecule that can carry foreign DNA into a host cell and replicate there

Cloning vector

9

Complementary DNA is resulting double stranded DNA made in virtro using mRNA as template for first strand

cDNA

10

Complementary molecule, a short single-stranded nucleic acid that can be either RNA or DNA

(Nucleic acid) probe

11

A brief electrical pulse applied to a solution containing cells creates in their plasma membrane in which DNA can enter

Electroporation

12

Technique where any specific target sequence can be quickly amplified ( copied many times) into test tube

PCR

13

Technique that uses a gel made if a polymer such as polysaccharide an acts as a sleeve to separate nucleic acids Or proteins basis of size, charge and properties

Gel electriohoresis

14

A method to detect and measure the expression of thousands of genes alone

DNA microarray

15

Method or silencing expression of selected genes

RNAi RNA interference

16

Produces one or more organisms identical to the parents

Cloning organisms

17

A cell with the potential for mature cells to dedifferentiate and five rise to speed

Totipotent

18

Is a relatively in specialized cell that can both reproduce itself indirect and under appropriate condition, differentiate unto specialized cells of one or more types

Stem cell

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Capable of differentiating into many different cells

Pluripotent

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Introducing genes into an afflicted individual for therapeutic reasons

Gene therapy

21

Replacement gene is packaged into virus and directly injected into patient

Direct devivery

22

Where the replacement gene is packaged into virus, which is used to infect stem cells and then stem cells transferred into body

Cell- based delivery