Chapter 3 (pages 45+)

Question 1

Its a nonpathogenic amoeba that has worldwide distribustion

Answer 1

endolimax nana

Question 2

where does the endolimax nana reside

Answer 2

humans large intestine

Question 3

size of endolimax nana trophozoites and cysts

Answer 3

cysts - 8-10 micrometer
trophozoites - 10-12 micrometer

Question 4

does endolimax nana cytoplasm have a chromatoid body or glycogen vacuole

Answer 4

False, it doesnt have a chromatoid body or glycogen vacuole

Question 5

what is the morphology of the karyosome of the nucleus of trophozoites and cysts of endolimax nana

Answer 5

eccentric and irregular

Question 6

it has worldwide distribution and less common than E. coli and E. nana

Answer 6

Iodamoeba butschlii

Question 7

size of Iodamoeba Butsclii’s trophozoite

Answer 7

12-15 micrometer

Question 8

size of Iodamoeba Butsclii’s cysts

Answer 8

10-12 micrometer

Question 9

true or false, the ectoplasm and endoplasm of the iodamoeba butschlii trophozoites are not differentiated

Answer 9

true

Question 10

true or false, the iodamoeba butschlii trophozoite’s, cytoplasm are not vacuolated

Answer 10

false, the cytoplasm ARE vacuolated

Question 11

true or false, the nucleus of the cyst of iodamoeba butschlii’s, central karyosome is surrounded by refractile chromatic granules.

Answer 11

true

Question 12

the cyroplasm of the cyst contains what? (iodamoeba butschlii)

Answer 12

large iodine stained glycogen mass or iodophilic body and no chromatic body

Question 13

diagnosis is done through what

Answer 13

stool examination

Question 14

What are used to differentiate species

Answer 14

Formalin ether/ethyl acetate concentratoin technique (FECT)

iodine stain

Question 15

how are trophozoites of E. gingavalis diagnosed

Answer 15

swab between gums

Question 16

how are cysts and throphozoites recovered

Answer 16

cysts - formed stools
trophozoites - watery or semi-formed stools

Question 17

how are trophozoites best demonstrated

Answer 17

direct fecal smear

Question 18

what techniques are used to recover from cysts

Answer 18

Formalin ether/ethyl acetate concentratoin technique

zinc sulfate flotation

Question 19

true or false. there is a treatment for these amoebae

Answer 19

false there is no treatments for these amoebae since they do not carry disease

Question 20

different ways to prevent and control amoebae

Answer 20

proper human disposal pf human waste

good personal hygiene

Question 21

single stool examinations of over 30,000 Filipinos, the prevalence of Entamoeba coli, Endolimax, and Iodamoeba butschlii

Answer 21

21%, 9%, and 1%

Question 22

Intestinal protozoan cysts were observed in ___ OFWs

Answer 22

13.5%

Question 23

How much of the food service workers in a hospital in Manila had Endolimax nana and E. Coli

Answer 23

20.3% and 13.6%

Question 24

How much of the food service workers in a HOSPITAL in Manila had Endolimax nana, E. Coli, E. hartmanni, and Iodamoeba butschlii

Answer 24

Endolimax nana - 22.8%
E. Coli - 17.9%
E. Hartmanni - 0 .8%
Iodamoebe Butscchlii - 0 .8%

Question 25

what pathogenic free-living-amoeba can cause human infections

Answer 25

Naegleria fowleri Acanthamoeba spp.

Question 26

Naegleria fowleri causes what

Answer 26

Primary amoebic meningoencephalitis

Question 27

Acanthamoeba spp. causes what

Answer 27

granulomatous amoebic encephalitis (GAE) and amoebic keratitis (AK).

Question 28

a thermophilic amoeba that thrives in warm water (e.g. hotsprings) and soil.

Answer 28

Naegleria fowleri

Question 29

what is the habitat of naegleria fowleri

Answer 29

Central Nervous System

Question 30

morphology of naegleria fowleri

Answer 30

1. Trophozoite (2 forms) (a) Amoeboid (b) Flagellate 2. Cyst

Question 31

naegleria fowler's trophozoite die at what chlorine level and salinity

Answer 31

2ppm and 0.7% salinity

Question 32

amoeboid form size and morphology of naegleria fowleri

Answer 32

10-20 micrometer and rounded pseudopodia, spherical nucleus, and big endosome.

Question 33

flagellate morphology of naegleria fowleri

Answer 33

biflagellated, pear shaped