Chapter 7

Question 2

MISSED CLASS ON 3/18

Answer 2

STARTED NOTES ON THIS SECTION AT 7.8

Question 3

Myoglobin: (4)

Answer 3

• monomeric heme protein
• found in tissues
• binds and releases O2 in tissues
• O2 storage

Question 4

Hemoglobin: (4)

Answer 4

• Tetrameric heme protein
• found in blood stream
• transports O2 from lungs/gills to peripheral tissues
• Returns CO2 to gills/lungs for exhalation

Question 5

Myoglobin vs Hemoglobin

Answer 5

SIMMILARITIES
- 8 a-helices
- helices names A through H

DIFFERENCES
- amino acid sequences

Question 6

REWATCH VIDEO TILL AT LEAST

Answer 6

SLIDE 33

Question 7

Ligand:

Answer 7

species that is available to bind

Question 8

Names!
Hb with O2
Mb with O2
—————————-
Hb with no O2
Mb with no O2

Answer 8

Hb - oxyhemoglobin
Mb - oxymyoglobin
———————————–
Hb - deoxyhemoglobin
Mb - deoxyhemoglobin

Question 9

Spectroscopic method: (2)

Answer 9

• can measure amount of hemoglobin present in body using color! (oxygenated is red, deoxy is purple)
• absorbance increases as O2 binds to hemoglobin

Question 10

Myoglobin’s ligand is

Answer 10

oxygen!

Question 11

Partial pressure of a gas is proportional ……..

Answer 11

to its concentration

Question 12

Oxygen binding curve for Myoglobin:
* x-axis:
* y-axis:
* Percent saturation is:

Answer 12

• partial pressure of O2 (PO2)
• fraction of sites occupied (YO2)
• percent of Mb that has O2 bound

Question 13

P50 is equal to:

Answer 13

PO (partial pressure) for half saturation

Question 14

P50 means:

Answer 14

half of all Mb is oxygenated

Question 15

POs in resting tissue is

Answer 15

~30 mm Hg

Question 16

Equation for hyperbolic curve of myglobin-O2 curve:

Answer 16

YO2 = PO2 / (P50 + PO2 )

Question 17

Low P50 =

Answer 17

lower concentration of O2 required

Question 18

High P50 =

Answer 18

greater concentration of O2 present