Chapter 7: Proteins Flashcards
(34 cards)
structure of an amino acid
-central carbon
-nitrogen (H3)
-carboxylic acid
-side chain (R group)
levels of structure
-primary (AA sequence)
-secondary (beta sheet, alpha helix)
-tertiary
-quaternary (more than 1 amino acid chain)
what causes proteins to denature
-heat
-pH
-enzymes
can we absorb denatured proteins
yes
what structures are lost in denaturing
-all but primary
what is sickle cell anemia
DNA mutation that codes for hemoglobin to become clumped (hydrophobic sides stick together, long cell not round)
what is transamination
-transfer amino group to new (non-essential) AA
-(B6 removes nitrogen group from AA)
deamination
-remove amino group
-excretion (in urea)
complete vs incomplete proteins
complete (high levels of all essential AA)
-animal protein
-quinoa and soy
-complementary proteins
incomplete (deficient in at lest one essential AA)
-plant proteins
Biological Value (BV)
ratio of N retained to N absorbed
how to find N retained
N absorbed - N urea = N retained
how to find N absorbed
N intake - N excreted = N absorbed
what is the N in urea
the proteins that have been used, discarding the N through the urea
what does BV measure
-quality: how efficiently body uses it
what is Protein efficiency ratio (PER)
-measures how well a protein supports growth in young animals (usually RATS)
-FDA uses for labeling regulations for infant food
nitrogen balance & mass
Negative nitrogen balance (losing mass)
-intake<losses
Nitrogen equilibrium
-Intake = losses
Positive nitrogen balance (gaining mass)
-intake>losses
protein RDA
.8g/kg body weight
how does pregnancy impact protein RDA
increased 25 g/d 2nd and 3rd trimester
how do americans do with protein
get plenty
what happens to excess protein (N & C)
the N is excreted, C skeletons used for energy
functions of proteins
-energy
-glucose formation
-acid base balance
-structure
-immune function (cells)
-hormones & enzymes
-fluid balance
how is protein digested
stomach
-HCl denatures protein
-pepsin breaks proteins into smaller pieces
small intestine
-chymotrypsin, trypsin, carboxypeptidase released and break things down more
-brush border enzymes (from microvilli) break things into AA
absorption
-intestinal cells absorb AA and small peptides
-large peptides are absorbed through endocytosis
how much of the body’s energy is from proteins
only 5-10% bc it’s inefficient
in prolonged exercise which AA are used for energy
-leucine
-isoleucine
-valine
bc they’re branched