Chemical and Enzymatic Methods Flashcards
(48 cards)
Chemical Methods include:
- ALKALINE COPPER REDUCTION METHOD
- ALKALINE FERRIC REDUCTION METHOD / HAGEDORN JENSEN
- CONDENSATION METHOD
ALKALINE COPPER REDUCTION METHOD includes:
FOLIN WU
NELSON SOMOGYI
NEOCUPROINE
BENEDICT’S
Reduce cupric ion to cuprous ion by the action of a reducing substance such as glucose
ALKALINE COPPER REDUCTION METHOD
Cu2+ + phosphomolybdic acid → phosphomolybdenum (blue)
FOLIN WU
Cu2+ + arsenomolybdic acid → phosphomolybdenum (blue)
NELSON SOMOGYI
Cu2+ + neocuproine → Cu – neocuproine (yellow/ y-orange)
NEOCUPROINE
end color of neocuproine
(yellow/ y-orange)
contains Cu(OH)2 copper hydroxide
Benedict’s reagent
end color of benedict’s
brick red precipitate
ALKALINE FERRIC REDUCTION METHOD aka
hagedorn jensen
ALKALINE FERRIC REDUCTION METHOD is _ method and _
TITRIMETRIC METHOD and INVERSE COLORIMETRY
Initial is colored and the final reaction is colorless.
Reduction of color is measured
INVERSE COLORIMETRY
in hagedorn jensen,
Potassium ferricyanide (yellow) is reduced when heated in an alkaline solution by a reducing sugar such as glcose, produces _ (colorless)
Potassium ferrocyanide
Uses: Ortho-toluidine is an aromatic amine
CONDENSATION METHOD
Method that uses glucose with aromatic amine forms schiff’s base when heated in an acidic sol’n
Absorbance:
Interferences:
Condensation Method
Absorbance: 630nm
Interferences: galactose & mannose can also form schiff’s base
Condensation Method’s acidic soln
glacial acetic
ENZYMATIC METHODS include:
- GLUCOSE OXIDASE METHOD
- HEXOKINASE METHOD
- GLYCOSYLATED HEMOGLOBIN METHODS
The most specific enzyme reacting with only B-D-Glucose
GLUCOSE OXIDASE METHOD
more abundant D-glucose in the blood
b-glucose (64%)
a-glucose in the blood
34%
Total Glucose also measures the a-glucose by adding the enzyme_ that will convert a-glucose to b-glucose.
Mutarotase
GLUCOSE OXIDASE METHOD falsely increase in
bleach
GLUCOSE OXIDASE METHOD falsely decrease in
ascorbic acid, uric acid, bilirubin, hemoglobin, tetracycline,
glutathione
GLUCOSE OXIDASE METHOD two methods:
COUPLED ENZYMATIC REACTION / SAIFER GERNSTENFIELD METHOD
POLAROGRPAHIC METHOD
