Chromatography

Question 1

1. Chromatography is the process for identification,
   purification and separation of components of a mixture on
   the basis of
   a) Difference in their boiling point
   b) Difference in their melting point
   c) Difference in their affinity for mobile and stationary
   phase
   d) Difference in their solubility

Answer 1

c) Difference in their affinity for mobile and stationary
phase

Question 2

2. Chromatography involves two mutually
   a) Immiscible phases
   b) Miscible phases
   c) Soluble phases
   d) None of the mentioned

Answer 2

a) Immiscible phases

Question 3

3. There is no need of support if packed into a column
   when the stationary phase is
   a) Gas
   b) Liquid
   c) Solid
   d) None of the mentioned

Answer 3

c) Solid

Question 3

4. Which of the following is not stationary phase?
   a) Liquid-liquid chromatography
   b) Gas-liquid chromatography
   c) Gas-solid chromatography
   d) Solid-solid chromatography

Answer 3

d) Solid-solid chromatography

Question 4

5. Liquid chromatography is a technique for separating
   a) Ions that are not dissolved in a solvent
   b) Ions that are dissolved in a solvent
   c) Ions that are dissolved in a solute
   d) All of the mentioned

Answer 4

b) Ions that are dissolved in a solvent

Question 5

7. The gas-solid chromatography is ______________
   chromatography as per basic principle involved.
   a) Exclusion
   b) Ion-exchange
   c) Adsorption
   d) Absorption

Answer 5

c) Adsorption

Question 5

6. In exclusion chromatography, solute molecules are
   separated based on
   a) Molecular geometry and size
   b) Molecular composition
   c) Molecular phase
   d) Molecular formula

Answer 5

a) Molecular geometry and size

Question 6

8. A proper solvent that is passed through the column for
   elution so that separated components can be collected is
   called
   a) Adsorbent
   b) Buffer solution
   c) Mobile phase
   d) None of the mentioned

Answer 6

b) Buffer solution

Question 7

9. The relative solubility of solute in both the phases
   determines the
   a) Rate of movement of solvent
   b) Rate of disappearance of solvent
   c) Rate of movement of solute
   d) Rate of disappearance of solute

Answer 7

c) Rate of movement of solute

Question 8

10. If the mobile phase is gas, movement of solute is
    determined by its
    a) Boiling point
    b) Melting point
    c) Solubility
    d) Volatility

Answer 8

d) Volatility

Question 9

1. Column chromatography is based on the principle of
   a) Ion-exchange
   b) Exclusion principle
   c) Differential adsorption
   d) Absorption

Answer 9

c) Differential adsorption

Question 10

2. Arrange the following compounds in order of their
   increasing adsorption tendencies.
   a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina
   b) Cellulose&raquo_space; starch&raquo_space; alumina&raquo_space; charcoal
   c) Charcoal&raquo_space; cellulose&raquo_space; alumina&raquo_space; starch
   d) Calcium carbonate&raquo_space;; alumina&raquo_space; starch&raquo_space; cellulose

Answer 10

a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina

Question 11

4. Select the correct statement from the following options.
   a) The lesser the polarity of solute, more strongly it will be
   adsorbed on a polar surface
   b) The greater the polarity of solute, more weakly it will be
   adsorbed on a polar surface
   c) The greater the polarity of solute, more strongly it will
   be adsorbed on a polar surface
   d) All of the mentioned option

Answer 11

c) The greater the polarity of solute, more strongly it will
be adsorbed on a polar surface

Question 11

3. What is the factor responsible for the separation in
   column chromatography?
   a) Polarity differences between the solvent
   b) Polarity differences between the solute
   c) Polarity indifference between the solvent
   d) Polarity indifference between the solute

Answer 11

b) Polarity differences between the solute

Question 12

5. The correct order of increasing strength of adsorption is
   a) Alkanes&raquo_space; Esters&raquo_space; Aldehydes&raquo_space; Phenols&raquo_space; Ketones
   b) Aldehydes&raquo_space; Phenols&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes
   c) Aldehydes&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes&raquo_space; Phenols
   d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

Answer 12

d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

Question 13

6. The components of the mixture in column
   chromatography are eluted in order of
   a) Increasing polarity and decreasing distribution ratio
   b) Increasing polarity and increasing distribution ratio
   c) Decreasing polarity and increasing distribution ratio
   d) Decreasing polarity and decreasing distribution ratio

Answer 13

b) Increasing polarity and increasing distribution ratio

Question 14

7. The elution power of a solvent is determined by
   a) Its overall polarity
   b) The polarity of the stationary phase
   c) The nature of the sample components
   d) All of the mentioned

Answer 14

d) All of the mentioned

Question 15

8. Which of the following is separated through column
   chromatography?
   a) Chlorophyll and carotenoids
   b) Inorganic cations or complexes
   c) Sugar derivatives
   d) Amino acids formed by hydrolysis of a protein molecule

Answer 15

a) Chlorophyll and carotenoids

Question 16

9. The mixture of petroleum ether and benzene is used in
   the elution ratio of
   a) 1 : 2
   b) 1 : 5
   c) 1 : 9
   d) 1 : 12

Answer 16

c) 1 : 9

Question 17

10. Chloroform fraction is eluted from the column by
    passing chloroform through the column which acts as
    a) Eluter
    b) Eluant
    c) Elution
    d) None of the mentioned option

Answer 17

b) Eluant

Question 18

1. The principle on which thin layer chromatography is
   based is that the
   a) Different compounds are absorbed on an absorbent to
   different degrees
   b) Different compounds are absorbed on an absorbent to
   same degrees
   c) Different compounds are adsorbed on an adsorbent to
   different degrees
   d) Different compounds are absorbed on an absorbent to
   same degrees

Answer 18

c) Different compounds are adsorbed on an adsorbent to
different degrees

Question 19

2. The size of a thin layer of adsorbent is about
   a) 0.1 mm
   b) 0.2 mm
   c) 0.3 mm
   d) 0.4 mm

Answer 19

b) 0.2 mm

Question 20

4. The eluant filled in the closed jar is
   a) Mixture of gases
   b) Mixture of a liquid and a gas
   c) Mixture of solids
   d) Mixture of liquids

Answer 20

d) Mixture of liquids

Question 21

5. Select the incorrect statement from the following
   options.
   a) The spots of colorless compounds are invisible to the
   eyes
   b) These spots can be detected by putting the plate under
   ultraviolet light
   c) These spots can be detected by placing the plate in a
   covered jar containing iodine crystals
   d) None of the mentioned

Answer 21

d) None of the mentioned

Question 22

6. The relative adsorption of each components of the mixture is expressed in terms of its a) Acceleration factor b) Retardation factor c) Both acceleration and retardation factor d) None of the mentioned

Answer 22

b) Retardation factor

Question 23

7. Select the correct statement from the following. a) Paper chromatography is a type of partition chromatography b) A special quality paper is used in paper chromatography c) Chromatography paper contains water trapped in it, which acts as stationary phase d) All of the mentioned

Answer 23

d) All of the mentioned

Question 24

3. The chromaplate or thin layer chromatography plate is made up of a) Glass b) Wood c) Fibre d) Metal

Answer 24

a) Glass

Question 25

8. Amino acids detected by spraying the plate with ninhydrin solution is an example of a) Column chromatography b) Thin layer chromatography c) Paper chromatography d) Liquid chromatography

Answer 25

c) Paper chromatography

Question 26

9. Paper chromatography is based on continuous differential partitioning of components of a mixture between stationary and mobile phases. a) True b) False

Answer 26

a) True

Question 27

10. Retardation factor is the ratio of a) Distance moved by substance from base line to distance moved by the solvent from base line b) Distance moved by solvent from base line to distance moved by the substance from base line c) Distance moved by substance from top line to distance moved by the solvent from top line d) Distance moved by solvent from top line to distance moved by the substance from top line

Answer 27

a) Distance moved by substance from base line to distance moved by the solvent from base line

Question 28

1. What are the uses of partition chromatography? a) Separation of amino acids b) Analysis of closely related aliphatic alcohols c) Separation of sugar derivatives d) All of the mentioned

Answer 28

d) All of the mentioned

Question 29

2. Which type of chromatography is used for the structural analysis? a) Column chromatography b) Paper chromatography c) Partition chromatography d) Affinity chromatography

Answer 29

b) Paper chromatography

Question 30

3. Which of the following is not done using column chromatography? a) The identification of unknown compounds b) The determination of homogeneity of chemical substances c) Separation of inorganic cations or complexes d) Separation of geometric isomers

Answer 30

c) Separation of inorganic cations or complexes

Question 31

4. Affinity chromatography is used for the analysis and isolation of a) Insoluble starch substances b) Enzyme tyrosinase c) Antibodies bound with a covalently-attached antigen on cellulose column d) All of the mentioned

Answer 31

d) All of the mentioned

Question 32

5. Purification of enzymes and proteins is done using a) Affinity chromatography b) Liquid chromatography c) Column chromatography d) Thin layer chromatography

Answer 32

a) Affinity chromatography

Question 33

6. Which of the following is not an application of high performance liquid chromatography? a) Analysis of proteins, drugs and explosives b) Separation of pharmaceutical drugs c) Elimination of undesirable substances from blood d) Separation of lipids, fatty acids and steroids

Answer 33

c) Elimination of undesirable substances from blood

Question 33

7. The checking of purity of samples is the application of thin layer chromatography. a) True b) False

Answer 33

a) True

Question 34

8. The analysis and separation of industrial products like soap and synthetic detergents is done using a) Thin layer chromatography b) Gas chromatography c) Ion exchange chromatography d) Partition chromatography

Answer 34

b) Gas chromatography

Question 35

9. Which of the following is the application of ion exchange chromatography? a) The softening of hard water b) The demineralisation of water c) The separation and determination of anions d) All of the mentioned

Answer 35

d) All of the mentioned

Question 36

10. The quantitative analysis is done using a) Ion exchange chromatography b) Thin layer chromatography c) Gas chromatography d) Liquid chromatography

Answer 36

c) Gas chromatography