Classification Flashcards
2 kingdoms proposed by?
linnaeus
6 kingdoms proposed by?
Woese
3 domain system includes?
- bacteria
- archaea
- eukarya
Bacterial nomenclature
Kingdom- phylum- class- order- family- genus- species- strain
Morphological characteristics of bacterial ID
- colony characteristics
- cell shape
- presence of capsule, endospore
- staining, G+ or G- or neither
- locomotion- presence of flagella and arrangment
biochemical testing?
test for utilization of different substrates like sugar
molecular taxonomy?
size of genome, G+C content, nucleic acid similarity, protein similarity
Diagnostic molecular pathology
created a labeled fluorescent probe ofknown DBA, apply probe to tissue to see if a match with complimentary stands
Polymerase Chain reaction?
produce millions of copies of target nucleic acid, if suspect bacterial or viral infection , attempt PCR on sample using mateial specific to infectious agent
analyze blood serum to search for specific antibodies present in serum, antibodies produced in response to specific bacerial antigens
immunological tests
serotype
an organism’s serotype indicates that it possesses a specific set of antigens
bacteriophage typing?
bacteriophage is a virus that infects a bacterium, phages are host specifc, if you can identify a phage which can infect a microbe you can deduce the bacteria involved