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Histopathology > Clearing > Flashcards

Clearing Flashcards

1
Q

clearing is also known as

A

dealcoholization

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2
Q

clearing agent must be ___ with the dehydrating agent, paraffin wax, and mounting medium

A

must be miscible

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3
Q

ideal amount of clearing

A

not less than 10x the volume

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4
Q

commonly used clearing agents

A
  • xylene
  • toluene
  • benzene
  • chloroform
  • cedarwood oil
  • aniline oil
  • clove oil
  • carbon tetrachloride
  • tetrahydrofuran
  • methyl benzoate/methyl salicylate
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5
Q

usual clearing time for xylene

A

1/2 - 2 hours

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6
Q

usual clearing time for toluene

A

1-2 hrs

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7
Q

urgent biopsies clearing time for xylene

A

15-30 mins

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8
Q

usual clearing time for benzene

A

15-60 mins

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9
Q

usual clearing time for chloroform

A

6-24 hrs

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10
Q

usual clearing time for cedarwood oil

A

2-3 days

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11
Q

most rapid clearing agent which can be used with celloidin sections

A

xylene

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12
Q

a substitute to xylene or benzene

A

toluene

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13
Q

rapid acting, recommended for urgent biopsies and routine purposes. it is carcinogenic and may damage bone marrow

A

benzene

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14
Q

best traditional clearing agents for routine use and gives the wides latitude

A

chloroform

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15
Q

recommended for tough (skin, fibroid, decalcified tissues) and large tissue specimens

A

chloroform

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16
Q

best for nervous tissue, lymph nodes, granulation tissue, feral, and other delicate highly cellular specimens

A

chloroform

17
Q

this clearing agent does not make tissues transparent and it is hepatotoxic after prolonged inhalation

A

chloroform

18
Q

clears both paraffin and celloidin sections

A

cedarwood oil

19
Q

this clearing agent recommended for CNS tissues and cytological studies esp. smooth muscles and skin

A

cedarwood oil

20
Q

recommended for clearing embryos and very delicate specimen

A

aniline oil

21
Q

removes aniline dyes and dissolves celloidin, tissue become brittle

A

clove oil

22
Q

properties are very similar to chloroform but it is cheaper

A

carbon tetrachloride

23
Q

it dehydrates and clears at the same time since it is miscible in both water and paraffin

A

tetrahydrofuran

24
Q

it can be used when double embedding technique are required

A

methyl benzoate/methyl salicylate

25
Q

ideal storage for specimen

A

1 month - 1 year

26
Q

ideal storage for serum/body fluids

A

48 hours

27
Q

ideal storage for pathology/bone marrow slides

A

10 years

28
Q

ideal storage for tissue blocks

A

10 yrs

29
Q

ideal storage for slides

A

indefinitely

30
Q

ideal storage for autopsy

A

indefinitely

31
Q

ideal routine turn over of results for surgical pathology and cytology

A

24 hrs

32
Q

ideal routine turn over of results for frozen section

A

5-15 mins

33
Q

ideal routine turn over of results for autopsy report

A

1 week

Histopathology (16 decks)

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