Clearing Flashcards
clearing is also known as
dealcoholization
clearing agent must be ___ with the dehydrating agent, paraffin wax, and mounting medium
must be miscible
ideal amount of clearing
not less than 10x the volume
commonly used clearing agents
- xylene
- toluene
- benzene
- chloroform
- cedarwood oil
- aniline oil
- clove oil
- carbon tetrachloride
- tetrahydrofuran
- methyl benzoate/methyl salicylate
usual clearing time for xylene
1/2 - 2 hours
usual clearing time for toluene
1-2 hrs
urgent biopsies clearing time for xylene
15-30 mins
usual clearing time for benzene
15-60 mins
usual clearing time for chloroform
6-24 hrs
usual clearing time for cedarwood oil
2-3 days
most rapid clearing agent which can be used with celloidin sections
xylene
a substitute to xylene or benzene
toluene
rapid acting, recommended for urgent biopsies and routine purposes. it is carcinogenic and may damage bone marrow
benzene
best traditional clearing agents for routine use and gives the wides latitude
chloroform
recommended for tough (skin, fibroid, decalcified tissues) and large tissue specimens
chloroform
best for nervous tissue, lymph nodes, granulation tissue, feral, and other delicate highly cellular specimens
chloroform
this clearing agent does not make tissues transparent and it is hepatotoxic after prolonged inhalation
chloroform
clears both paraffin and celloidin sections
cedarwood oil
this clearing agent recommended for CNS tissues and cytological studies esp. smooth muscles and skin
cedarwood oil
recommended for clearing embryos and very delicate specimen
aniline oil
removes aniline dyes and dissolves celloidin, tissue become brittle
clove oil
properties are very similar to chloroform but it is cheaper
carbon tetrachloride
it dehydrates and clears at the same time since it is miscible in both water and paraffin
tetrahydrofuran
it can be used when double embedding technique are required
methyl benzoate/methyl salicylate
