Embedding

Question 1

Embedding is also known as?

Answer 1

Blocking or casting

Question 2

process by which a tissue is arrange in a precise position in the mold during embedding, on the microtome before cutting, and on the slide before staining

Answer 2

orientation

Question 3

embedded tissues will then be immersed in a melted paraffin at a temp between ____

Answer 3

5-10C above its melting point

Question 4

after immersing the tissue in the melted paraffin, what is the next step?

Answer 4

cooled in a ref at -5C or immersed in cold water to solidify

Question 5

enumerate the types of blocking-out molds

LCPD

Answer 5

• Leukhart’s embedding mold
• Compound embedding mold
• Plastic embedding rings with base mold
• Disposable embedding molds

Question 6

this type consists of 2 L-shaped pieces of heavy brass or metal, etc.

Answer 6

Leukhart’s embedding mold

Question 7

this type is made up of a series of interlocking plates resting on a flat metal base, forming several compartments

Answer 7

Compound embedding mold

Question 8

this type consist of a special stainless steel base mold fitted with a plastic embedding ring which later serves as the block holder during cutting

Answer 8

Plastic embedding rings with base mold

Question 9

give an example of plastic embedding rings with base mold

instrument

Answer 9

tissue tek

Question 10

enumerate the disposable embedding molds

PPP

Answer 10

• Peel-aways
• Plastic ice trays
• Paper boat

Question 11

used in ordinary refrigerators recommended for busy routine labs

Answer 11

plastic ice trays

Question 12

cheap and easy to make used for embedding celloidin and paraffin blocks

Answer 12

paper boat

Question 13

enumerate the other embedding methods

Answer 13

• Celloidin/Nitrocellulose method
• Double embedding method
• Plastic resin method

Question 14

this method is recommended for embedding hard tissues such as bones and teeth and for large sections of organs like eyes

Answer 14

Celloidin/Nitrocellulose method

Question 15

low viscosity nitrocellulose is a form of celloidin soluble in equal concentration of?

Answer 15

ether and ethanol

Question 16

the process in which tissues are first infiltrated with celloidin and subsequently embedded in paraffin mass

Answer 16

double embedding method

Question 17

double embedding method is used to facilitate cutting of large blocks of firm tissues such as ___ and small sections of ___

Answer 17

large blocks of firm tissues such as brain and small sections of celloidin blocks

Question 18

this method has superior results for** light microscopic studie**s (hard tissues - undecalcified bone) and for high resolution light microscopy of tissue sections (renal and bone marrow biopsies)

Answer 18

Plastic resin method

Question 19

enumerate the plastic resins

Answer 19

• epoxy plastics
• polyester plastics
• acrylic plastics

Question 20

this plastic resin is recommended for EM

Answer 20

epoxy plastics