Lec Staining

Question 1

Give the specific stains and what it stains

Answer 1

Perl’s Prussian blue- hemosiderin (hemoglobin)
Periodic Acid Schiff- polysaccharides (carbohydrates)

Question 2

Give the stains in direct/simple staining

Answer 2

Methylene blue
Eosin

Question 3

Examples of indirect staining

Answer 3

Mordant
Accentuator

Question 4

Examples of mordant

Answer 4

Potassium alum with hematoxylin in Ehrlich’s hematoxylin
Iron in weigert’s hematoxylin

Question 5

Examples of accentuators (2)

Answer 5

KOH in Loeffler’s methylene blue
Phenol in carbon thionine and carbol fuchsin

Question 6

Stains used in progressive staining

Answer 6

Gill’s haematoxylin
Mayer’s haematoxylin

Question 7

Stains used in regressive staining

Answer 7

Harris’ haematoxylin
Delafield’s haematoxylin

Question 8

This staining does not stain nucleus

Answer 8

Vital staining

Question 9

mixture of PICRIC ACID and ACID FUCHSIN for the demonstration of CT, MUCIN, and ELASTIC TISSUE

Answer 9

Van Gieson’s (Acid Fuschin PIcric Acid)

Question 10

basic acridine flurochrome which permits discrimination between DEAD AND LIVING cells, giving GREEN fluorescence for DNA

Answer 10

Acridine Orange

Question 11

for CALCIUM SALTS and PHOSPHATASE ACTIVITY

Answer 11

Acridine Red 3B

Question 12

CYTOPLASMIC STAIN used for COUNTERSTAINING of EPITHELIAL CELLS

Answer 12

Aniline Blue

Question 13

stain ACID MUCOPOLYSACCHARIDES, more specific for CT and EPITHELIAL MUCIN

Answer 13

Alcian Blue

Question 14

a PLASMA STAIN utilized for deep staining of acid fast organisms’ MITOCHONDRIA, and a differentiation of SMOOTH MUSCLES with the use of PICRIC ACID

Answer 14

Basic Fuchsin
(Carbol fuchsin, Coleman’s Feulgen, Schiff’s, Mallory’s fuchsin, Aldehyde fuchsin/Gomori’s)

Question 15

staining HAEMOGLOBIN

Answer 15

Benzidine

Question 16

stains DIPTHERIA ; used for contrast stain for Gram’s technique, acid-fast, and Papanicolaou method

Answer 16

Bismarck Brown

Question 17

used as a CHROMATIN stain for fresh materials in smear preparations

Answer 17

Carmine

Question 18

combined with ALUMINUM CHLORIDE to stain GLYCOGEN

Answer 18

Best Carmine

Question 19

a mordanted dye acting as a basic dye and staining ACID SUBSTANCES

Answer 19

Mayer’s Carmalum solution

Question 20

recommended for staining of FIXED SECTIONS

Answer 20

Celestine Blue

Question 21

best known as INDICATOR; may be used as a stain for axis cylinders in EMBRYOS; used for staining ELASTIC TISSUES, AMYLOID, and MYELIN (Krajian’s Method)

Answer 21

Congo Red

Question 22

a NUCLEAR or CHROMATIN stain used for staining AMYLOID in FROZEN SECTIONS and PLATELETS

Answer 22

Crsytal Violet

Question 23

mixture of CV, Methyl Violet, and Dextrin

Answer 23

Gentian Violet

Question 24

stain blood to DIFFERENTIATE LEUKOCYTES

Answer 24

Giemsa

Question 25

used for **METALLIC IMPREGNATION**, made up of *gold chloride* and *mercuric chloride*

Answer 25

Gold Sublimate

Question 26

the **OLDEST** and stains **AMYLOID**, **CELLULOSE**, **STARCH**, **CAROTENES**, and **GLYCOGEN**

Answer 26

Gram's Iodine and Lugol's Iodine

Question 27

used for staining **MITOCHONDRIA**

Answer 27

Janus Green B

Question 28

*contrast stain* for **ASCARIS EGGS** and **ERYTHROCYTES**, and **BACTERIAL SPORE** stain

Answer 28

Malachite Green

Question 29

stains **CHROMATIN** *GREEN* when an acid is present

Answer 29

Methyl Green

Question 30

DIPTHERIA diagnosis

Answer 30

Methylene Blue

Question 31

stains **PLASMA CELLS**, fresh **SPUTUM** for malignant cells, *evaluation and differentiation* of **bacterial organisms**

Answer 31

Methylene Blue

Question 32

coloring **NUCLEI of LEUKOCYTES** *REDDISH-PURPLE* in presence of *METHYLENE BLUE*

Answer 32

Methylene Violet

Question 33

for observing **CELL GRANULES** and **VACUOLES** of *phagocytic cells*

Answer 33

Neutral Red

Question 34

**substitute for carbol fuchsin** in acid-fast staining

Answer 34

Night Blue

Question 35

an excellent stain for **ELASTIC FIBERS**; demonstrates the *finest and most delicate fibers in skin* used for **DERMATOLOGIC STUDIES**

Answer 35

Orcein

Question 36

commonly used as a **fixative** and is used to stain **FAT**

Answer 36

Osmium Tetroxide

Question 37

utilized for manufacture of **PAINTS** used as a **MICROANATOMICAL** *contrast stain* for demonstration of the *circulatory system* by injection (intravital staining)

Answer 37

Prussian Blue

Question 38

used with *osmic acid* to fix and stain **BLOOD** and **GLANDULAR TISSUES**

Answer 38

Rhodamine B

Question 39

used in identification of **SPIROCHETES**, **RETICULUM**, and OTHER **FIBER STAINS**

Answer 39

Silver Nitrate

Question 40

stains **NISSL** granules or **CHROMOPHILIC BODIES** and *nuclear stain for FIXED TISSUES*

Answer 40

Toluidine Blue

Question 41

used as a **substitute for thionine** in f*resh tissue sections*

Answer 41

Toluidine Blue

Question 42

stains **NEUROGLIA** in *frozen section*

Answer 42

Victoria Blue

Question 43

enumerate basic fuchsin stains

Answer 43

Schiff’s Carbol fuchsin Coleman’s Feulgen Aldehyde fuchsin/Gomori’s Mallory’s fuchsin

Question 44

Enumerate cytoplasmic counterstains

Answer 44

RED Eosin Y Eosin B Phloxine B Rose Bengal YELLOW Picric acid Orange G GREEN Light green SF Lissamine green

Question 45

Enumerate nuclear counterstain

Answer 45

RED Neutral red Safranin Carmine BLUE Methylene blue Toluidine blue Celestine blue Hematoxylin

Question 46

What is Trypan Blue

Answer 46

Used in vital staining of reticuloendothelial system

Question 47

This staining demonstrates cytoplasmic phagocytosis

Answer 47

Vital staining

Question 48

Used to stain living cells immediately after removal from the living body

Answer 48

Supravital staining

Question 49

Done by injecting the dye into any part of the animal body (IV, subcutaneous, intraperitoneal) producing specific coloration of certain cells, particularly those of the RES

Answer 49

Intravital staining

Question 50

Used in supravital staining and is probably the best vital dye

Answer 50

Neutral red

Question 51

Stains used in intravital staining

Answer 51

Lithium India ink Carmine

Question 52

Metallic impregnation have the greatest application in tissue from the ___ and for the demonstration of ___

Answer 52

CNS, reticulin

Question 53

Stains used in metallic impregnation

Answer 53

Silver nitrate Osmium tetroxide

Question 54

This is the most commonly used stain in metallic impregnation

Answer 54

Silver nitrate

Question 55

This staining entails the use of the specific dyes that stains tissues with a color that is different from that of the stain color itself

Answer 55

Metachromatic staining (metachromasia)

Question 56

Stains used in metachromasia

Answer 56

Metachromatic dye Cresyl blue

Question 57

This stain is a basic dye belonging to Thizine and Triphenylmethane groups which stains for **mast cell granules**

Answer 57

Metachromatic dye

Question 58

Stain used for reticulocytes which stains it violetish-blue or deep blue

Answer 58

Cresyl blue

Question 59

Stains under histochemical staining

Answer 59

Perl Prussian Blue Periodic Acid Schiff