CLEARING

Question 1

Process whereby alcohol or dehydrating agent is removed from tissue and replaced with substance that will dissolve wax

Answer 1

Clearing

De-alcoholization

Question 2

Most commonly used clearing agent for de-alcoholization in the embeddinv process are:

Answer 2

Xylene
Dioxane
Chloroform
Cedarwood

Question 3

Used when the tissue is to be cleared directly from water as in a frozen section

Answer 3

Glycerin

Gum syrup

Question 4

Make the tissue transparent due to high refractive index for better visualization

Answer 4

Clearinh agents

Question 5

Steps requiring complete clearing

Answer 5

Infilltration

Mounting

Question 6

Generally suitable for most routine histologic processing shedules <24hrs <5mm thickness

Answer 6

Xylene

Question 7

Miscible with alcohol and paraffin

Answer 7

Xylene

Toluene

Question 8

Doesnt extract out aniline dyes

Answer 8

Xylene

Question 9

Used for celloidin section

Answer 9

Xylene

Question 10

Becomes milky when an incompletely dehydrated tissue is immersed in

Answer 10

Xylene

Question 11

Substitute for xylene and benzene but slower

Answer 11

Toluene

Question 12

Best clearing agent for embedding

Answer 12

Benzene

Question 13

Can damage the bone marrow resulting to aplastic anemia

Answer 13

Benzene

Question 14

Thick blocks up to 1 cm can be processed with this clearing agent

Answer 14

Chloroform

Question 15

Clearing agent doesnt not become translucent

Doesnt make tissue transparent

Answer 15

Chloroform

Question 16

Recommended for tough tissues

Answer 16

Chloroform

Question 17

Toxic to the liver after prolonged inhalation

Answer 17

Chloroform

Question 18

Vapor attack the rubber seal

Answer 18

Chloroform

Question 19

For both celloidin and paraffin

Answer 19

Cedarwood oil

Question 20

Tissue with cedarwood oil initially float and may dry out before completely cleared

Prevented by?

Answer 20

Superimposing absolute alcohol of the surface of clearing agent

Question 21

For clearing embryos, insects, delicate specimen

Answer 21

Aniline oil

Question 22

Clear 70% alcohol without excessive shrinkage and hardening

Answer 22

Aniline oil

Question 23

Tendency to become adulterated

Answer 23

Clove oil

Question 24

Similar with chloroform but cheaper

Answer 24

Carbon tetrachloride

Question 25

Slow acting clearing agents Used when double embedding is required

Answer 25

Methyl benzoate Methyl salicylate Amyl acetate

Question 26

Should not be used for nervous tissues and lymph nodes

Answer 26

Xylene

Question 27

Fast acting for urgent biopsies

Answer 27

Xylene | Benzene

Question 28

Tends to acidify partially filled vessel

Answer 28

Toluene

Question 29

Tissues tend to float with this clearing agent

Answer 29

Chloroform

Question 30

Extremely slow

Answer 30

Cedarwood oil

Question 31

Becomes milky when prolonged storage

Answer 31

Cedarwood oil

Question 32

For CNS and cytological studies

Answer 32

Cedarwood oil

Question 33

Slow and difficult | Expensive

Answer 33

Clove oil

Question 34

Faint pleasant odor

Answer 34

Terpineol

Question 35

Substitute for cedarwood oil

Answer 35

Terpineol

Question 36

For clearing skin and smooth muscle

Answer 36

Oil of bergamot

Question 37

Use for delicate tissue

Answer 37

Oil of wintergreen | Aniline oil

Question 38

Xylene substitute

Answer 38

Toluene (xylene and benzene) N-butyl acetate Limonene

Question 39

Nitrocellulose solvent

Answer 39

N-butyl acetate Amyl acetate Methyl salicylate Methyl benzoate

Question 40

Obtained form citrus fruits

Answer 40

Limonene