DEHYDRATION

Question 1

The process of removing intercellular and extracellular water from the tissue following fixation and prior to wax impregnation

Answer 1

DEHYDRATION

Question 2

Embryonic tissues starts dehydration at what percent

Answer 2

30% ethanol

Question 3

Alcohol recommended for routine dehydration

Answer 3

Ethyl alcohol

Question 4

Best dehydrating agent

Fast acting

Answer 4

Ethyl alcohol

Question 5

Toxic dehydrating agent

Answer 5

Methyl alcohol
Dioxane
Cellosolve
Tetrahydrofuran

Question 6

For blood and tissue films

Smear preparation

Answer 6

Methyl alcohol

Question 7

Utilized in plant and animal micro-techniques

Answer 7

Butyl alcohol

Question 8

Slow dehydrating agent

Answer 8

Butyl alcohol

Question 9

Formalin fixed tissue must be transferred directly to what alcohol?

Answer 9

Higher grade of alcohol 85-95% alcohol

Question 10

It tends to harden only the surface of the tissue while deeper parts are not completely penetrated

Answer 10

Concentrated alcohol

95% to absolute

Question 11

To avoid hardening of the surface only use?

Answer 11

70% gradually increased to 95% is used

Question 12

It tends to macerate the tissue

Answer 12

Prolonged storage in below 70% alcohol

Question 13

Temperature that hasten dehydration time

Used for urgent exam

Answer 13

37C

Question 14

For fragmentary biopsies

Answer 14

Temp 37C

Question 15

What to do to ensure complete dehydration

This will accelerate dehydration by removing water from the dehydrating fluid

Answer 15

Layer of anhydrous copper

Sulfate 1/4 inch deep covered with filtered paper

Question 16

It is an indication of full saturation of dehydrating fluid with water

Answer 16

Blue discolorization of copper sulfate crystals

Question 17

More miscible with epoxy resins than alcohol

Answer 17

Acetone

Question 18

Highliy flammable

Answer 18

Acetone

Question 19

Rapid action but penetrates tissue poorly

Answer 19

Acetone

Question 20

Lipids are remove from the tissue with this dehydrating agent

Answer 20

Acetone

Question 21

Other term of dioxane

Answer 21

Diethylene dioxide

Question 22

Dehydrating and clearing agent

Answer 22

Dioxane

Tetrahydrofuran

Question 23

It tends to ribbon poorly

Answer 23

Dioxane

Question 24

Method that tissue is wrapped in gauze bag and suspended with dioxane and anhydrous calcium oxide

Answer 24

Weiseberger’s method

Question 25

Dehydrates rapidly

Answer 25

Cellosolve

Question 26

Cellosolve

Answer 26

Ethylene glycol monoethyl ether

Question 27

Ethylene glycol ether is combustible in what temp?

Answer 27

110-120F

Question 28

Used to dehydrate section and smears

Answer 28

Triethyl phosphate

Question 29

THF

Answer 29

Tetrahydrofuran

Question 30

``` Miscible with Water Melted paraffin Alcohol Xylol ```

Answer 30

Dioxane

Question 31

``` Miscible in water and paraffin Lower alcohols Ether Chloroform Acetone Benzene Xylene ```

Answer 31

Tetrahydrofuran

Question 32

May be used for demixing, Clearing, Dehydrating paraffin section

Answer 32

THF

Question 33

Hard tissues can be immersed in

Answer 33

Glycerol Alcohol mixture or in MOLLIFLEX

Question 34

Tissues can be left with this reagent for long period of time without affecting the consistency and staining properties

Answer 34

Dioxane

Question 35

Two types of dioxane

Answer 35

Graupners | Weiseberger

Question 36

Only to small pieces

Answer 36

Acetone

Question 37

Indicator of h2o saturation

Answer 37

Anhydrous copper sulfate