FIXATIVES

Question 1

Satisfactory for routine paraffin section

Answer 1

Aldehyde fixatives

Question 2

It allow the natural colors to be restored for one hour

Answer 2

70% alcohol

Question 3

Methods of removing formalin pigment (brown)

Answer 3

Kardasewitch
Lillies
Picric acid

Question 4

Prolonged storage of formaldehyde produce what?

Answer 4

Precipitation of white paraformaldehyde

Turbidity

Question 5

White paraformaldehyde precipitates may be remove tru?

Answer 5

Add 10% methanol

Question 6

Bleaching of tissues may be prevented by

Answer 6

Changing the fluid fixatives for 3 months

Question 7

If fatty tissues are to be stored

What is added to prevent dispersion of fat into the fluid

Answer 7

Cadmium

Cobalt

Question 8

Acid reaction due to formic acid formation may be nuetralized by adding?

Answer 8

Magnesium carbonate

Calcium carbonate

Question 9

Added to buffer formalin but produces calcium deposits

Answer 9

Calcium acetate

Question 10

For general post mortem tissue

Answer 10

10% formol saline

Question 11

Surgical post mortem

Answer 11

10% NBF

Question 12

Routine post mortem tissues

Answer 12

Formal corrosive

Question 13

Silver impregnation

Answer 13

10% formol saline

Heidenhain’s susa

Question 14

Best fixative do tissue containing iron pigments and elastic fibers

Answer 14

10% NBF

Question 15

Silver reticulum method

Answer 15

Formal corrosive

Question 16

Does not allow frozen tissue section

Answer 16

Formal corrosive

Question 17

For glycogen and micro-incineration

Fix sputum

Coagulates mucus

Answer 17

Alcohol formalin

Question 18

For enzyme histochem and EM

Answer 18

Glutaraldehyde

Question 19

Trichrome staining excellent

Answer 19

Mercuric chloride

Question 20

Routine fixative choice for tissue photography

Answer 20

Mercuric chloride

Question 21

Recommended for renal tissues, fibrin, CT, muscles

Answer 21

Mercuric chloride

Question 22

Mercuric deposits may be removed by

Answer 22

Saturated iodine solution in 96% alcohol

Alcoholic iodine solution

Question 23

Mercuric chloride added by glacial acetic acid

Answer 23

Zenker’s

Question 24

Mercuric chloride added strong formaldehyde

Answer 24

Zenker formol

Helly’s

Question 25

It is added to the zenker fluid to prevent turbidity and formation of black precipitates

Answer 25

Glacial acetic acid

Question 26

For liver

Answer 26

Zenkers fluid

Question 27

For pituitary gland

Answer 27

Zenker formol

Question 28

For tumor biopsies

Answer 28

Heidenhain susa

Question 29

For bone marrow biopsies

Answer 29

B5

Question 30

Preserves carbohydrate

Answer 30

Chromic acid

Question 31

Preserves mitochondria

Answer 31

P. Dichromate

Question 32

For demonstration of chromatin Mitochondria Golgi bodies RBC and colliod containing tissues

Answer 32

Regauds | Moller

Question 33

For tissue necrosis Rickettsiae Myelin

Answer 33

Orths fluid

Question 34

For acid mucopolysaccharides

Answer 34

Lead fixatives

Question 35

For demonstration of glycogen

Answer 35

Picric acid

Question 36

Removing of yellow color produced by picricacid

Answer 36

70% ethyl alcohol Followed 5% sodium thiosulfate Washed in running water

Question 37

For embryos and pituitary biopsies

Answer 37

Bouins soln

Question 38

Endometrial curettings

Answer 38

Bouins

Question 39

Not suitable for fixing kidneys

Answer 39

Bouins

Question 40

Messy | Excellent Fixative for glycogen

Answer 40

Brasils

Question 41

Solidifies at 17C

Answer 41

Glacial acetic acid

Question 42

Cause glycogen polarization

Answer 42

Alcohol fixatives

Question 43

Excellent for fixing dry and wet smear

Answer 43

Methyl alcohol 100%

Question 44

For fixing touch prep

Answer 44

Isopropyl alcohol 95%

Question 45

70-100 % concentration

Answer 45

Ethyl alcohol

Question 46

For lymph glands | Chromosomes

Answer 46

Carnoys

Question 47

For diagnosis of rabies

Answer 47

Carnoys | Acetone

Question 48

Most rapod fixative

Answer 48

Carnoys

Question 49

For mucopolysaccaridea and nuclear proteins

Answer 49

Newcomers

Question 50

Better reaction with feulgen stain

Answer 50

Newcomers

Question 51

Strong oxidizing solution

Answer 51

Osmium tetroxide

Question 52

Brilliant nuclear staining with safranin

Answer 52

Osmium tetroxide

Question 53

Depresses the staining power of hematoxylin

Answer 53

Flemming

Question 54

For phosphatases and lipases

Answer 54

Acetone

Question 55

For em

Answer 55

Glutaraldehyde Osmic Paraformaldehyde