component prep

Question 1

is a mixture of cells that can be
separated into different components

Answer 1

Whole Blood

Question 2

In WB, PLT is viable for_____ and Factor Vand VIII ____

Answer 2

In WB, PLT is viable for 1 day and Factor V
and VIII decreases

Question 3

SINGLE blood bag is for

Answer 3

WB,
PRBC(open)

Question 4

DOUBLE blood bag is for

Answer 4

WB, RRBC, FFP

Question 5

TRIPLE

Answer 5

WB, RRBC,
FFP, PC

Question 6

QUADRUPLE

Answer 6

WB, RRBC, FFP,
PC, CP, Plasma
Derivatives

Question 7

volume of whole blood

Answer 7

VOLUME-450 mL of donor blood and anticoagulant solution

Question 8

storage and shelf life for whole blood

Answer 8

 STORAGE- +2 to +6 deg Celsius
 PRESERVATIVE: CPDA-1, CPD,ACD
 SHELF-LIFE: 35 days , 21 days

Question 9

Minimum of___ of transfused Red cells should survive 24-hours post
transfusion

Answer 9

Minimum of 70% of transfused Red cells should survive 24-hours post
transfusion

Question 10

Indicated for patients who are actively bleeding,

Answer 10

WHOLE BLOOD

Question 11

lost >25% of Blood
volume,

Answer 11

WHOLE BLOOD

Question 12

exchange transfusion

Answer 12

WHOLE BLOOD

Question 13

Indicated for Anemic or Hemolytic patients without loss of
Blood volume:

Answer 13

PACKED RED BLOOD CELLS

Question 14

for Oncology patients,

Answer 14

prbc

Question 15

Trauma Victims,

Answer 15

PACKED RED BLOOD CELLS

Question 16

Surgical
Patients,

Answer 16

PACKED RED BLOOD CELLS

Question 17

End-stage renal diseases,

Answer 17

PACKED RED BLOOD CELLS

Question 18

Sickle Cell Disease

Answer 18

prbc

Question 19

Two types of Preparation of PRBC

Answer 19

 Sedimented (60-70% RBC)
 Centrifuged (70-80% RBC)

Question 20

PACKED RED BLOOD CELLS

 Steps in separation

Answer 20

1. Place bag in buckets of Refrigerated Centrifuge. Make sure components are balanced
2. Centrifuge at 5000xg for 5 mins (Hard Spin) at 2-6 dec
3. Express 3⁄4 of the plasma into the satellite bag using plasma expressor
4. Seal tube after expressing

Question 21

PRBC VOLUME, HCT, HGB, PH, HEMOLYSIS

Answer 21

Volume: 250-300
Hematocrit: 55-75
Hemoglobin: >45g/dL
pH: 6.4-7.4
Hemolysis <0.8%

Question 22

Residual Leukocyte count OF PRBC

Answer 22

Residual Leukocyte count <2x10^9/uL

Question 23

Platelet Concentrate VOLUME, TEMP AND STORAGE

Answer 23

Volume 50-70mL
Temp: +20 to +24 deg C
Storage: 5 days with constant agitation

Question 24

bleeding problems or
bleeding tendencies, or as prophylaxis

Answer 24

Platelet Concentrate

Question 25

PLATELET CONCENTRATE CAN BE SOURCED FROM

Answer 25

Could be sourced from Allogeneic Blood (RD) or Apheresis (SD)

Question 26

PLATELET CONCENTRATE STEPS IN PREP

Answer 26

Steps in separation 1. Blood should be less than 6 hours and stored at 20-22 deg C 2 . Centrifuge bag at 2000xg for 3 mins (SOFT SPIN) at 20-24 deg C 3. Express approx. 4/5 Platelet Rich Plasma (PRP) in the satellite bag. Seal the tube after expressing 4. Centrifuge PRP in HARD SPIN for 7 mins 5. Express Platelet Poor Plasma (PPP) into another satellite bag. Leave approx. 50 mL of plasma 6. Rest PC for 1 hour 7. Store PC at 20-22 under constant agitation

Question 27

CONCENTRATION OF PLATELETS

Answer 27

Concentration: 5.5x10^10/uL (3x10^11/uL if apheresis)

Question 29

RESIDUAL LEUKOCYTE, RBC COUNT AND PH OF PLATELET CONCENTRATE

Answer 29

Residual Leukocyte count <1x109/ul Residual RBC count <1x109/ul pH 6.4-7.4 (should never fall below 6)

Question 30

VOL, SHELF LIFE AND STORAGE OF FFP

Answer 30

Volume: 200 mL Shelf Life: 1 year Storage: -30 deg C

Question 31

Coagulation factor deficiency, Volume replacement, DIC, Severe Liver diseases

Answer 31

FFP

Question 31

ffp step prep

Answer 31

Steps in Separation (NO PC required) Blood should collected within 8 hours Centrifuge WB in HARD SPIN Express approx. 4/5th of plasma in the satellite bag Label the plasma bag and rapidly frozen Thaw in the refrigerator or water bath

Question 32

quality check for ffp

Answer 32

Quality check Volume 180-200 ml Factor VIII level: >0.7 iu/mL Fibrinogen level >300 mg/unit

Question 33

CRYOPRECIPITATE Component Characteristics

Answer 33

Component Characteristics Volume <15ml Storage: -30 or colder Shelf Life: 1 year

Question 34

Indicated for patient in need of Firbrinogen or VWF or used as FibrinGlue in surgical Settings

Answer 34

CRYOPRECIPITATE

Question 35

CRYOPRECIPITATE Steps in Separation

Answer 35

Steps in Separation Thaw FFP at 40C until it is liquid Centrifuge Hard Spin Express supernatant into a satellite bag and label as Cryo-poor plasma Freeze within 1 hour

Question 36

CRYOPRECIPITATE Quality check

Answer 36

Quality check Volume: 10-25 mL AHF Activity: 80 units Fibrinogen: 150 mg