Compound Development Techniques Flashcards
Outcome parameters for cancer trials
Tumour growth and survival of animals
Outcome parameters for Meningitis
Bacterial burden, neurological signs, survival
Haemophilia outcome measures
Bleeding events, blood clotting, thrombin generation assay
Diabetes outcome measures
Blood Glucose, glucose tolerance, insulin sensitivity
How to induce diabetes
Alloxan to destroy pancreatic B cells
How to induce haemophilia
FVIII or FIX KO
How to induce cancer
Xenograft
How to test if a compound is anti inflammatory
Use a model using LPS to stimulate inflammation or carrageenan in vivo
reduction of that
How to test if a compound is anti-depressant
Forced swim test
How to separate compounds from an unknown mix
Thin layer chromatography to separate compounds
How does thin layer chromatography work
Separates compounds based on their solubility and affinity for the stationary phase
How to test which separated compounds are active
Try each compound in a cell culture which a define outcome criteria
How to find the structure of the active compound
Use mass spec to seperate compound into ions using mass to charge ratio
Then ions present can be used to work out what the compound is
How to develop a drug
Find a pathway that can be modulated
Develop a compound to modulate it
Test it based on define efficacy criteria in cells
Then in animals
Then in humans if it is safe and effective
