Culture Media in Diagnostic Microbiology (51-104) Flashcards

(53 cards)

1
Q

Selective and Differential

isolation and differentiation of lactose and non-lactose fermenting Gr (-) bacilli

A

MacConkey Agar

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2
Q

Selective and Differential

isolation and differential of mannitol and non-mannitol fermenting Staphylococci

A

Mannitol Salt Agar (MSA)

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3
Q

Selective

isolation of pathogenic Neisseria sps. like N. gonorrhoeae

A

Martin Lewis Agar

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4
Q

Selective

isolation of Listeria monocytogenes

A

McBride Medium

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5
Q

Selective

isolation of Mycobacterium species

A
Middlebrook 7H9 (liquid)
7H10/7H11 (solid)
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6
Q

Selective

isolation of Mycobacterium species

A

Mitchison Agar

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7
Q

Antibiotic Susceptibility Test Medium

AST of most bacteria

A

Mueller-Hinton Agar/broth

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8
Q

Tissue Cell Culture

isolation of Chlamydia

A

McCoy cells

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9
Q

Biochemical Characteristic Medium

identification of Clostridium perfringes

A

Litmus Milk Medium

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10
Q

Biochemical Characteristic Medium

identification of Gr (-) enteric bacilli based on ability to produce LDC/ODC/ADH

A

Moeller’s Broth

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11
Q

Biochemical Characteristic Medium

differentiation of organisms based on their ability to oxidize or ferment carbohydrates

A

Oxidation-Fermentation (O-F) medium

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12
Q

Selective

isolation of Neisseria spp.

A

Modified Thayer Martin Medium

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13
Q

Selective

isolation of pathogenic sps. of Neisseria like N. gonorrhoeae and also Mycoplasma hominis and Ureaplasma urealyticum

A

New York City Agar

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14
Q

Selective

isolation of Mycobacterium spp.

A

Dorset Egg Medium

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15
Q

Selective

isolation of Mycobacterium spp.

A

Petragnani Medium

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16
Q

Selective

isolation of Mycobacterium spp.

A

American Thoracic Society Medium

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17
Q

Selective

isolation of non tuberculous Mycobacteria like M. avium

A

Wallenstein Medium

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18
Q

Selective

selective isolation of gram (+) cocci and anaerobic gram (-) bacilli

A

Phenylethanol Agar

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19
Q

Selective and Enrichment

isolation of Bordetella pertussis

A

Regan Lowe Agar

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20
Q

Selective and Differential

isolation and differentiation of Salmonella and Shigella

A

Salmonella-Shigella Agar

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21
Q

Nonselective Medium

recovery of aerobes and anaerobes

A

Schaedler Agar

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22
Q

Enrichment

enrichment and isolation of Salmonella spp.

A

Selenite F Broth

23
Q

Selective

isolation of Campylobacter spp.

A

Skirrow’s Medium

24
Q

Selective

isolation of S. pyogenes and S. agalactiae

A

Streptococcal Selective Agar

25
Differential differentiating non-fermentative bacilli like Pseudomonas, Acinetobacter, Alkaligenes
Seller's Agar
26
Biphasic Medium blood culture
Scott's Modified Castañeda and Thioglycollate Broth
27
Transport maintenance of viability of microorganisms
Stuart's Medium
28
Selective isolation of Mycoplasma spp.
Shepard's A7-B Agar
29
Enrichment isolation of Salmonella and Shigella spp from stools
Tetrathionate Broth
30
Selective isolation of pathogenic spp of Neisseria
Thayer Martin Agar
31
General Supportive recovery of aerobes, anaerobes, microaerophilic, and fastidious microorganisms
Thioglycollate Broth
32
Selective isolation of Corynebacterium diphtheriae
Tinsdale Agar
33
Selective and Differential isolation and differentiation of Vibrio spp.
Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)
34
Enrichment and Selective enrichment and isolation of Streptococcus agalactiae
Todd-Hewitt Broth supplemented with antibiotics
35
General Purpose Medium supports the growth of non-fastidious and fastidious bacteria; can be used as a base in preparing blood agar
Trypticase Soy Agar/Broth
36
Selective Transport Neisseria gonorrhoeae
Transgrow
37
Biochemical Characteristic Medium identification of Gr (-) enteric bacilli based on their ability to produce indole
Tryptone Broth
38
Biochemical Characteristic Medium identification of Gr (-) enteric bacilli based on their ability to (1) ferment glucose, lactose, and sucrose; (2) produce gas and hydrogen sulfide
Triple Sugar Iron Agar
39
Biochemical Characteristic Medium identification of Gr (-) enteric bacilli based on their ability to ferment glucose via mixed acid fermentation or butylene glycol pathway
Methyl Red Voges Proskauer Broth (MRVP) or | Peptone Glucose Broth
40
Biochemical Characteristic Medium identification of Gr (-) enteric bacilli based on their ability to produce enzyme urease
Christensen Urea Agar or | Stuart Urea Broth
41
Biochemical Characteristic Medium identification of Gr (-) enteric bacilli based on their ability to produce enzyme deaminase
Phenylalanine Deaminase Agar (PDA)
42
Biochemical Characteristic Medium identification if Gr (-) enteric bacilli based on their ability to utilize citrate as source of carbon
Simmon's Citrate Agar
43
Differential Medium identification of organisms based in their ability to grow or not to grow in the presence of 6.5% NaCl
Salt (NaCl) Broth
44
Biochemical Characteristic Medium differentiate beta-hemolytic streptococci and enterococci based on their ability to produce enzyme, pyrrolidonylarylamidase or pyroglutamylaminopeptidase
PYR Agar
45
Selective isolate and identify Pseudomonas aeruginosa
Cetrimide Agar Slant
46
Biochemical Characteristic Medium identification of organisms which utilize acetate as a source of carbon like Escherichia coli
Acetate Slant
47
Biochemical Characteristic Medium identification of organisms which utilize acetamide as a source of carbon like P. aeruginosa
Acetamide Slant
48
Selective and Differential identification of Gr (-) enteric bacilli and for the differentiation of Salmonella and Shigella spp.
Xylose Lysine Desoxycholate Agar
49
Selective identification of Corynebacterium diphtheriae
Pai's Agar
50
Selective identification of Corynebacterium diphtheriae
McLeod's Agar
51
Tissue Cell Culture isolation of Rickettsia
HeLa Cell Line
52
Tissue Cell Culture isolation of Rickettsia
Embryonated Egg
53
Employed in Animal Inoculation isolation and identification of Mycobacterium leprae
Foot Pad of Mice and Armadillo