Haemophilus and Other Fastidious Bacteria

Question 1

blood lover

Gr (-), small, pleomorphic coccobacilli

Answer 1

Haemophilus

Question 2

most require X (hemin) and V (NAD) factors except H. aphrophilus

Answer 2

Haemophilus

Question 3

has satellite growth

Answer 3

Haemophilus

Question 4

Pfeiffer’s Bacillus

Answer 4

Haemophilus influenzae

Question 5

major pathogen and frequently associated during flu pandemics

Answer 5

Haemophilus influenzae

Question 6

has the ff virulence factors:

• capsule (serotyping)
• IgA protease
• fimbriae/pili
• outer membrane proteins and LPS

Answer 6

Haemophilus influenzae

Question 7

Ducrey’s Bacillus

Answer 7

Haemophilus ducreyi

Question 8

strict human pathogen;

female sex workers

Answer 8

Haemophilus ducreyi

Question 9

causes Chancroid or Soft Sore

Answer 9

Haemophilus ducreyi

Question 10

Gram stain looks similar to a school of fish

Answer 10

Haemophilus ducreyi

Question 11

Haemophilus influenzae biogroup III;

• non-encapsulated
• Pediatric conjuctivitis
• Brazillian purpuric fever

Answer 11

Haemophilus influenzae biogroup aegyptius

Question 12

Koch-Weeks Bacillus

Answer 12

Haemophilus aegyptius

Question 13

causes pink eye (highly contagious conjuctivitis)

Answer 13

Haemophilus aegyptius

Question 14

What staining is done to diagnose Haemophilus?

Answer 14

Acridine orange staining

Question 15

Streaking done for primary isolation and satellite growth

Answer 15

Staph streak

Question 16

Nairobi plate is for

Answer 16

H. ducreyi

Question 17

It is a clear medium with X and V factors

Answer 17

Levinthal Agar

Question 18

Delta-aminolevulinic acid test is also known as

Answer 18

Porphyrin test

Question 19

The porphyrin test’s (+) result is

Answer 19

red fluorescence (brick red)

Question 20

susceptible to penicillin and has a bipolar staining of sefety pin appearance

Answer 20

Pasteurella multocida

Question 21

has a mushroom smell/musty odor

Answer 21

Pasteurella multocida

Question 22

Shipping Fever in cattles

Answer 22

Pasteurellosis

Question 23

Bang’s Bacillus

Answer 23

Brucella

Question 24

Gram (-) bacilli/coccobacilli has sand appearance

Answer 24

Brucella

Question 25

It induces spontaneous abortions and sytemic disease

Answer 25

Brucella

Question 26

requires *niacin* for growth

Answer 26

Brucella abortus

Question 27

It is the gold standard for Brucella

Answer 27

​Serum Agglutination Test (detection of febrile agglutinins)

Question 28

Facultative intracellular pathogen. Causative agent of Tularemia (Rabbit, Deerfly, Lemming, Water Rat Tapper's fevers)

Answer 28

Francisella tularensis

Question 29

signs & symptoms include: * ulceroglandular (most common; ulcers) * pneumonic (most serious form) * oculoglandular (conjuctivitis and lymphadenopathy) * typhoidal (no skin lesions)

Answer 29

Francisella tularensis

Question 30

causes waterborne tularemia and less virulent

Answer 30

Francisella philomiragia

Question 31

Bordet Gengou Bacillus; capsulated; DTaP (Diphtheria, Tetanus, Pertussis)

Answer 31

Bordetella pertussis

Question 32

causative agent of whooping cough or pertussis (highly contagious)

Answer 32

Bordetella pertussis

Question 33

causes kennel cough among animals

Answer 33

Bordetella bronchiseptica

Question 34

petussis-like illness

Answer 34

Bordetella holmesii

Question 35

preferred staining for Bordetella

Answer 35

Direct Fluorescent Antibody stain (+) yellow green fluorescence

Question 36

Bordetella pertussis is incubated at?

Answer 36

35 degrees Celsius for 7 days in Bordet Gengou Potato Infusion Agar

Question 37

all are sodium hippurate hydrolysis (except *L. pneumophila*)

Answer 37

Legionella

Question 38

no recorded/documented person to person infection

Answer 38

Legionella

Question 39

most clinically significant species; possess 2 well-defined, morphologically distinct forms in HeLa cells

Answer 39

Legionella pneumophila

Question 40

Pontiac fever is associated with

Answer 40

Legionnaires disease

Question 41

causes Pittsburgh pneumonia

Answer 41

Legionella micdadei

Question 42

causes Wiga agent pneumonia

Answer 42

Legionella bozemanii

Question 43

causes pneumonia

Answer 43

Legionella dumoffi

Question 44

sputum is treated with ____ sol'n

Answer 44

KCl:HCl

Question 45

What is HACEK?

Answer 45

***_H_**aemophilus spp.* ***_A_**ggregatibacter* ***_C_**ardiobacterium hominis* ***_E_**ikenella corrodens* ***_K_**ingella kingae*

Question 46

loves high levels of CO2; does not require X and V factor

Answer 46

Aggregatibacter aphrophilus

Question 47

lactose fermenters that has a grade school paste odor

Answer 47

Aggregatibacter aphrophilus

Question 48

presence of a star shape with 4-6 points in the center of colonies growing in BHI agar after 48 hrs

Answer 48

Aggregatibacter actinomycetemcomitans

Question 49

virulence factors: * collagenase * leukotoxin

Answer 49

Aggregatibacter actinomycetemcomitans

Question 50

has a rosette formation, teardrop, dumbell, lollipop appearance

Answer 50

Cardiobacterium hominis

Question 51

capnophilic that has a bleach-like odor

Answer 51

Eikenella corrodens

Question 52

B-hemolytic on BAP catalase (-) indole (-)

Answer 52

Kingella kingae

Question 53

can be mistaken for *N. gonorrhoeae* on **Modified Thayer Martin Agar**

Answer 53

Kingella denitrificans