Cutting and Joining DNA

Question 1

Acts as a scissor to cut DNA at a specific site

Answer 1

Restriction Enzyme

Question 2

Another term for “Restriction Enzyme”

Answer 2

Restriction Endonuclease

Question 3

Glue that joins 2 DNA molecules

Answer 3

DNA Ligase

Question 4

Cut in the same position, and nucleotides are left unpaired. Produces a straight and equal cut.

Answer 4

Blunt end

Question 5

Cut in a different position. Produces a zigzag and uneven cut

Answer 5

Sticky end

Question 6

Commonly used type of enzyme for cutting DNA at specific sequences.

Answer 6

EcoRI, HindIII, BamHI, etc.

Question 7

What is the purpose of DNA ligation in molecular biology techniques?

Answer 7

Joining DNA fragments

Question 8

The process of cutting DNA at specific sequences using restriction enzymes?

Answer 8

Restriction digestion

Question 9

The process of introducing foreign DNA into host organisms

Answer 9

Transformation

Question 10

A piece of DNA that is isolated from the desired organism and inserted into a vector for cloning where it combines with vector DNA

Answer 10

Foreign DNA or Passenger DNA