Cloning Vector Flashcards

1
Q

Serves as a vehicle that transports foreign DNA into host cells

A

Cloning Vector

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2
Q

A molecule in which foreign DNA can be inserted to produce clones of recombinant DNA

A

Cloning Vector

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3
Q

Where the trait is located

A

Gene locus

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4
Q

DNA replication occurs in?

A

Nucleus

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5
Q

ORI (site) stands for?

A

Origin of replication initiation

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6
Q

The starting point of DNA replication

A

ORI site

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7
Q

Cloning vectors should be _________ for efficient transformation and stable maintenance of inserted DNA.

A

small enough

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8
Q

A cloning vector should have unique ____________ where it will be cut.

A

restriction sites

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9
Q

____________ are used to determine wanted genes, for them to be able to survive. These are mostly antibiotic resistance genes.

A

selectable markers

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10
Q

Original double-stranded DNA remains intact, while a completely new copy is synthesized.

A

Conservative replication

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11
Q

DNA strands break and rejoin in a scattered manner, resulting in segments of original and newly synthesized DNA.

A

Dispersive replication

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12
Q

Each strand of the original DNA molecule serves as a template for the synthesis of a new complementary strand.

A

Semi-conservative replication

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13
Q

The three models of replication are:

A

Conservative, Dispersive, Semi-conservative

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14
Q

Prokaryotes have ___ site of replication

A

only one

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15
Q

Eukaryotes have ________ of replication.

A

multiple sites

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16
Q
A