DNA Cloning

Question 1

A molecular biology technique that makes many identical copies of DNA

Answer 1

DNA cloning

Question 2

A commonly used vector in DNA cloning

Answer 2

Plasmid

Question 3

This step involves extraction of DNA from the source organism.

Answer 3

(1) Isolation of DNA (genome)

Question 4

This step uses restriction enzyme and DNA ligase to create compatible ends with the DNA fragment.

Answer 4

(2) Ligating the DNA into a vector (plasmid)

Question 5

In this step, The host cell takes up and incorporates the DNA and the target gene is inserted into a circular piece of DNA (plasmid)

Answer 5

(3) Transformation of a host cell with the recombinant DNA (reproduce)

Question 6

This step Identifies cells with successfully integrated recombinant DNA.

Answer 6

(4) Selection of host cells with the recombinant DNA. (wanted gene insert)

Question 7

In this step, Tests are conducted to confirm the success of DNA cloning.

Answer 7

(5) Screening for Recombinant DNA

Question 8

Artificially formed DNA by combining components from different organisms

Answer 8

Recombinant DNA

Question 9

First step in DNA cloning

Answer 9

Isolation of DNA

Question 10

Second step in DNA cloning

Answer 10

Ligating DNA into a Vector

Question 11

Third step in DNA cloning

Answer 11

Transformation of Host Cell

Question 12

Fourth step in DNA cloning

Answer 12

Selection of Host Cells

Question 13

Fifth step in DNA cloning

Answer 13

Screening for Recombinant DNA

Question 14

Sixth step in DNA cloning

Answer 14

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