Day 4

Question 1

What hematological determination is used to classify anemia’s based on the morphological characteristics of the RBCs?

Answer 1

RBCs Indices

Question 2

What does the MCV tell you about the RBCs?

Answer 2

Indicates the size of the RBC

Question 3

What does the MCH tell you about the RBCs?

Answer 3

weight(mass) of hemoglobin

Question 4

What does the MCHC tell you about the RBCs?

Answer 4

Tells what percentage of RBCs is hemoglobin

Question 5

What does the RDW tell you about the RBCs?

Answer 5

Degree of anisocytosis is present, or degree of variability of area cell

Question 6

What is the unit of measure for MCV?

Answer 6

Femtoliters (fl)

Question 7

What is the unit of measure for MCH?

Answer 7

Picograms (pg)

Question 8

What is the unit of measure for MCHC?

Answer 8

g/dl or percentage (%)

Question 9

What is the unit of measure for RDW?

Answer 9

Percent(%)

Question 10

What is the calculation for MCV?

Answer 10

MCV= HCT% x 10
———————
RBC count

Question 11

What is the calculation for MCH?

Answer 11

MCH= HGB g/dl x 10
————————-
RBC count

Question 12

What is the calculation for MCHC?

Answer 12

MCHC= HGB(g/dL) x 100
—————————-
HCT

Question 13

What is the calculation for RDW?

Answer 13

RDW= SD OF MCV x 100
——————-
Mean MCV

Question 14

Which RBC indice is the most reliable automated index?

Answer 14

Mean Corpuscular Volume (MCV)

Question 15

Which RBC indice is the most effective discriminant for classification of anemia’s?

Answer 15

Mean Corpuscular Volume (MCV)

Question 16

Name one disease state in which one many have a MCV of 60-70 fl?

Answer 16

Iron Deficiency Anemia

Question 17

What is the normal range for MCH?

Answer 17

27-33pg

Question 18

What does MCHC valve of 40+ indicate?

Answer 18

malfunction or error in manual calculation

Question 19

Which RBC indice is the least reliable in automated methods?

Answer 19

Mean Corpuscular Hemoglobin Concentration (MCHC)

Question 20

What is the reference range of RDW?

Answer 20

11-15%

Question 21

What blood film method is used by NCCLS as a reference method?

Answer 21

Wedge Blood Film( blood smear)

Question 22

What is the purpose of Methanol in the Wright-Giemsa stain?

Answer 22

Kills and fixes cels

Question 23

What type of dyes are used in the Wright-Giemsa stain?

Answer 23

Basic and acidic aniline dyes

Question 24

Explain the pH and staining characteristics of Wright-Giemsa stain.

Answer 24

Cells with high pH(base) stain blue(basophils)

Cells with low pH(acid) stain orange(eosinophils)

Question 25

Explain what the RBCs and Monocytes should look like Ifthe Wright-Giemsa stained properly?

Answer 25

RBCs should be pink to red-orange | Monocytes should have light purple nuclei; cytoplasm should be blue-gray

Question 26

Explain what the cells will look like if the stain is too basic.

Answer 26

Cells would be overstained, giving an overall blue effect(including RBCs); nuclear chromatin will stain dark blue to black.

Question 27

Explain what would happe if stain is too acidic.

Answer 27

RBCs too red, while WBCs and platelets appear pale.

Question 28

In the Wright-Giemsa stain, how do granules stain?

Answer 28

Bright orange in eosinophils and dark blue in basophils

Question 29

In the Wright-Giemsa stain, how do platelets stain?

Answer 29

Violet to purple

Question 30

In the Wright-Giemsa stain, how should lymphocytes stain?

Answer 30

Dark purple nuclei and cytoplasm should be clear to robins egg blue

Question 31

In the Wright-Giemsa stain, how should neutrophils stain?

Answer 31

Dark purple nuclei and cytoplasm should be light pink with lilac granules

Question 32

What is the reference range for MCV?

Answer 32

80-96 fl

Question 33

RDW measurement is derived from automated cell counters that ____________ measure the MCV.

Answer 33

Directly

Question 34

The MCHC only increases in _________________; it is decreased or remains normal in other anemia’s.

Answer 34

Spherocytosis

Question 35

What is indicated in a MCHC below 32?

Answer 35

Hypochromasia

Question 36

What is the MCHC limit for hemoglobin solubility?

Answer 36

37 g/dL

Question 37

MCH may be as high as ____ in macrocycytic anemia.

Answer 37

50pg

Question 38

MCH may be as low as ____ or les in microcytic anemias

Answer 38

20pg

Question 39

Other than MCV , what other indice is a reliable automated index?

Answer 39

MCH

Question 40

In what type of anemia may the MCV be up to 150 fl?

Answer 40

Pernicious anemia