Deck 7

Question 1

Principle of Virus neutralisation test (VNT):

Answer 1

Highly specific test based on the neutralization of virus by specific antibodies in the serum tested.
- Cytopathic effect of the virus in cell culture is blocked.
- Performed in tubes or microtitre plates.
- Positive serum contains specific antibodies that neutralized virus and therefore the virus cannot damage the cells (CPE).
- Negative serum, no antibodies, virus not neutralized and can cause cytopathic effect.

Question 2

PCR components ?

Answer 2

Template DNA, nucleotides, primers, buffers, DNA polymerase, thermocycler.

Question 3

Rabies way of transmission:

Answer 3

Primarily through saliva, bite wounds or infected saliva entering an open cut or wound or mucus membrane. Sources of virus is saliva and brain tissue. Occasionally by infected aerosol or oral.

Question 4

ParaTB transmission ?

Answer 4

Faecal-oral.

Question 5

Listeriosis is ?

Answer 5

Alimentary.

Question 6

What is OIE ?

Answer 6

World health organization for animal health.

Question 7

Culture methods:

Answer 7

Monolayer
Suspension
Microcarrier

Question 8

Reservoir of Aujeszky ?

Answer 8

Swine.

Question 9

Culture of fungi ?

Answer 9

Saubourads.

Question 10

Footrot aetiological agent ?

Answer 10

Bacteriosus nodosus and Fusobacterium necrophorum.

Question 11

Samples for African Swine Fever (ASF) ?

Answer 11

Blood from liver, spleen, lymph node, tonsil and kidney from dead.

Question 12

Aetiological agent of Rabies ?

Answer 12

Lyssavirus
Classical Rabies Virus (RABV)

Question 13

Rapid slide agglutination best for Brucellosis ?
As well as used for Salmonella.

Answer 13

Rose bengal.

Question 14

PCR test is used for detection of ?

Answer 14

Genome part of cell nucleus.

Question 15

Staphylococci are ?

Answer 15

Gram positive.