Deck 9 Flashcards
(15 cards)
What are main reagent of PCR ?
Primers, enzyme (Taq DNA polymerase), DNA sample, dNTP.
Source of infection is ?
Macroorganism, in which etiological agent surives, multiplies and is excreted.
What is the principle of haemagglutination test ?
To inhibit agglutination activity of some viruses such as parvo, parainfluenza and adeno.
Division of the media used for cell culture ?
Natural, synthetic.
Complement fixation test is used for detection of ?
Antibody.
In sandwich ELISA positive results is ?
Change of colour.
What is tropism of pathogens ?
Monotropic
Pantropic
Polytropic
Name at least 3 tests for cellular immunity evaluation:
Flow cytometry, complete blood count and lymphocyte proliferation assay.
What is the role of colostral immunity ?
To provide passive immunity and protection against septicemia and organ infection as well as local protection of intestine and GIT bacteria multiplication.
Clinical signs indicating failure of passive transfer of immunity:
Profuse diarrhoea, dehydration, respiratory or systemic infections.
In blocking ELISA, positive result is ?
Change of colour.
Example of facultactive pathogens:
Candida
Acanthamoeba
Animal notifiable diseases are reported to ?
World health organization for animal health (OIE).
What are ways of etiological agents transmission ?
Direct contact, vector, vehicle, air-borne.
What test is virological ?
Virus neutralization.
