Diagnosis

Question 1

Bluetongue

Answer 1

– clinical evaluation
– postmortem examination
– serologic testing
– viral identification

Question 2

Bovine Ephemeral Fever

Answer 2

– Cx and Lx
– postmortem examination
– PCR viral identification

Question 3

Bovine Spongiform Encephalopathy

Answer 3

– ELISA
– Western Immunoblot Techniques
– Immunohistochemical Techniques

Question 4

Bovine Leukosis

Answer 4

– Serologic Testing
– Cytology or histologic exam / biopsy

Question 5

Bovine Petechial Fever

Answer 5

– based on CSx and histopathologic examination of samples
– definitive Dx requires identification of E. ondiri

Question 6

Bovine Papular Stomatitis

Answer 6

– Cx and demo of virions by electron microscopy of lesion scrapings

Question 7

Bovine Respiratory Disease Complex

Answer 7

– PE, CSx, necropsy, bacterial isolation
– molecular identification of viral infections

Question 8

Bovine Viral Diarrhea - Mucosal Disease Complex

Answer 8

– CSx and Lx
– Virus isolation
– Serology
– PCR

Question 9

Caprine Arthritis and Encephalitis (CAE)

Answer 9

– CSx and Hx
– Serologic testing (AGID and ELISA)

Question 10

Contagious Ecthyma

Answer 10

– ecthyma lesion is characteristic
– demonstration of virus by electron microscopy

Question 11

FMD

Answer 11

– RT-PCR assay, serology, or virus isolation

Question 12

Maedi-visna

Answer 12

– ELISA or agar gel immunodiffusion (AGID) assay

Question 13

Malignant Catarrhal Fever

Answer 13

– clinical evaluation, PCR assay

Question 14

Warts

Answer 14

– clinical examination, histopathology, PCR, Southern blot, dot blot, reverse blot, in situ hybridization and immunohistochemistry

Question 15

Rabies

Answer 15

– Immunofluorescence microscopy on fresh brain tiss.
– Molecular testing