Diagnostics Flashcards
(83 cards)
CMT
tests milk for mastitis - somatic cell count
4 wells for 4 quarters
more gel formation = more scc
rated 0-3 - 3 most severe
quick, done at cow side, inexpensive, uncomplicated
results a little subjective
can detect subclinical mastitis
IgG Measurement in neonates
measure of passive transfer of antibodies to neonates - blood total protein, SNAP test
identity neonates at risk of failure of passive transfer
below 10 for dairy calves or 24 for beef calves considered failure of passive transfer
also colostrum testing - 50 minimum for good quality, under 20 very bad
brix refractometer or colostrometer
FEC cut offs
sheep -
nematodirus - any
other roundworms - 200
lungworm - doesn’t usually detect - if any then def treat
fluke - 200
cocci - 2500 - 1000 not super unusual
cows -
roundworm - 200
tapeworm - not reliable, probably treat if you see bits of one in there
fluke - 200
cocci - 2500 - 1000 not super unusual
lungworm - doesn’t usually detect - if any then def treat
pig -
roundworm - 200
tapeworm - not reliable, probably treat if you see bits of one in there - also main tapeworm is a nasty human one
fluke - 200
cocci - 2500 - 1000 not super unusual
lungworm - doesn’t usually detect - if any then def treat
bulk milk tank tests
tb
johnes
bvd
somatic cell count
ketosis/acidosis
fluke
lepto neospora
schmallenberg
to assess nutrition
pm samples - sheep - abortion
lamb - placenta, abortion materials
placenta -
toxo - strawberry cotyledons
chlamydia (enzootid abortion) - thickened between cotyledons
brucella - necrosis and oedema - leathery
campylobacter - vasculitis and bacterial colonies
lamb -
mummification - toxo and chlamydia most common
deformity - schmallenberg (not notifiable), border disease (like bvd equiv in sheep - persisent infected lambs - reportable), nutritional deficiencies, toxo, various bacterial infections
samples for PCR for infectious disease
pm samples - sheep - sudden death
clostridial disease - black disease, pulpy kidney - gas in tissues, unusually rapid and resolving rigor mortis, also gassy distention of organs
pulpy kidney - kidney decomposition quicker then other organs and are soft and easy to break
black disease - rapid decomposition, black skin discolouration, liver necrosis
lamb dysentery - jejunem - emphysema, haemorrage (dark red, distended, ulcerated)
redgut - intestinal torsion (lush pastures)
plant toxins - may still be in mouth or rumen
parasites - parasites themselves visible in gut, liver or lungs, cysts visible, scarring, fecal sample from GI for eggs
pm samples - cow - sudden death
pneumonia - discoloured consolidated areas in lung, viral may be more subtle than bacteria
pasteurella - severely congested lungs with petechial haemorrhage
blackleg - rapid breakdown of affected muscle, sweet smell
bloat - gas distention of rumen, with or without obstruction in oesophagus
toxin - mauy be in mouth or rumen, may have liver or kidney damage
lightening strike - scorch marks
pm samples - cow abortion
calves -
planta -
retained - can be result of dystocia, metritis, twin births, management issues
placentitis - redness? - usually bacterial infection (metritis)
brucella - yellowish, and moroccan leather (thick grey yello) around cotyledons
chlamydia - yellow blown exudate and thickening
toxicoses - discoloured exudative cotyledons, thickening and oedema between cotyledons
foetus -
brain - vit e/selenium
fluid from abdomen or thorax - serological tests - bvd etc
thyroid - iodine
parasites
congenital conditions
toxin ingestion - may still be in stomach
pm samples - pig sudden death
intestinal torsion
gastric tilation and torsion
e coli - usually post weaning - oedema in brain and abdomen
glassers - can be fatal in young pigs - red/purple discolouration in ears, abdomen and legs, joint swelling
clostridium noyvi - gangrene, gas, inflammation in tissues
pm checks - chicken
feet - pododermatitis - welfare, immune system
newcastles - lungs - inflammation, congestion
parasites - cocci - intestinal scarring, thickening, presence of the parasites themselves
AI - hemorrhage, oedema necrosis - various organs
mareks
mareks - grey white patches of neoplastic material - lungs, spleen, liver, heart, skeletal muscle
pm - pig - abortion
brucella - piglet - necrosis and white foci on liver, sometimes liver, spleen, brain
CSF - enlarged spleen and liver. widespread petechial hemorrhage
ASF - enlarged spleen and liver. widespread petechial hemorrhage, button ulcers in large intestine
prrs - lung lesions, mummification, still birth, PCR for the virus
porcine parvo -chalklike deposits on placenta and placenta oedema, mummified stillborn or premature foetus,
tb - pm signs
caseous circle type lesions - liver and lungs
enlarged lymph nodes (usually front of the cow)
may be too small to see or similar to lesions of other disease - PCR on material from them
condemned if lesions in more than one organ
johnes - pm signs
thickened ileum wall
caseous or calcified lymph nodes
may be condemned but no strict rule
pneumonia - pm signs
heavy lungs
consolidation
exudate - fibrinous or purulent
edema (fluid accumulation) or empysema (excessive air in lung tissue)
lymph node enlargement -
mycoplasma - grey/purple consolidated lungs
viral tends to be cranial, bacterial caudal
key notifiable disease
AI
newcastles
bTB
Foot and mouth
anthrax
brucella
BSE
Scrapie
Bluetongue
sheep scab (SCOTLAND)
classical and african swine fever
swine vesicular disease
rabies
scours
lambs and calves - fecal sample to differentaite pathogen - ELISA - negative if too early
FEC
pig - PCR fecal sample - same idea
also - litmus fecal sample - ecoli tends to be alkali, viral acidic
johnes -
respiratory
johnes
post mortem - thickened intestinal wall, enlarged lymph nodes, bacteria visible on histopath with ZN stain
fecal culture - more accurate than pcr but longer time, still only any good if shedding
fecal PCR - quick but only if shedding
pooled or individual fecal samples
herdsure - uses blood elisa -2 tests a year apart for better security
always risk not shedding or not produced antibodies, routine testing and retesting best practice
tb
tuberculin skin test
interferon gamma test - blood - confirm skin test
notifiable - how often need to test depends on status
cull if confirmed
carcass downgraded
condemned if lesions in more than one body part
schmallenberg signs
adult - reduced yield, fever, recover quickly
foetus/neonate -
abortion or stillbirth
deformities - bent limbs, twisted neck or spine, domed skill, brain abnormalities
if alive - small, weak, deformed, blind
nervous signs - inability to suckle, convulsions
recumbancy
schemmelnberg pm
calf -
brain - underdeveloped, small
limbs - fixed in position
spine - scoliosis (curve)
foetal monster
schmallenberg testing
not notifiable in uk
pm - brainstem and foetal fluid - send away for ELISA, with details of deformity
fec reduction values
90-95% less as aim
false negatives at fec
intermittent shedding
immature parasites - prepatent period
encysting
single sex infection
liver fluke - sometimes present without eggs in feces
lungworm - usually present without eggs in feces
sample issues - too old, too small, not stored right, not taken across a sample group
recent treatment prompting a temporary drop
other veterinary treatment - eg steroids
fecal abnormalities - can be affected by scour or starvatoin