DNA EXTRACTION Flashcards
(15 cards)
1
Q
how many mL of specimen is transfered to a 1.5 mL microcentrifuge tube
A
1
2
Q
volume of EL buffer used
A
100 uL
3
Q
how many minutes is gram (+) bacteria incubated at 37’C
A
30-60 mins
4
Q
how many minutes is gram (-) bacteria incubated at 37’C
A
10-15 minutes
5
Q
volume of RS buffer used
A
100 uL
6
Q
volume of PK used
A
10 uL
7
Q
at what temp is the solution incubated after adding 10 uL of PK
A
56’C for 15 minutes
8
Q
volume of GA buffer used
A
200 uL
9
Q
at what KG is the solution centrifuged after adding 200 uL of GA buffer
A
13,000 KG for 1 minute
10
Q
at what KG is the solution centrifuged after adding 500 uL of G Binding buffer
A
10,000 KG for minute
11
Q
volume of BA buffer used
A
400 uL
12
Q
volume of G Binding buffer
A
500 uL
13
Q
volume of wash buffer
A
500 uL
14
Q
volume of elution buffer used
A
100 uL
15
Q
A
