DNA extraction methods

Question 1

a procedure used to isolate DNA from the nucleus of cells

Answer 1

dna extraction

Question 2

dna extraction was done first by who?

Answer 2

friedrich miescher

Question 3

lysis of cell and the nucleus to extract DNA to the buffer

Answer 3

cell dissolution

Question 4

removing the impurities and proteins from the sample

Answer 4

precipitation

Question 5

final stage and to get a completely pure DNA sample

Answer 5

purification

Question 6

basically the isolation of nucleic acid material

Answer 6

dna extraction

Question 7

method is based on typical chromatographic methods

Answer 7

chromatography-based extraction method

Question 8

DNA molecules and other simple and complex molecules in a COMPLEX MATRIX can be separated based on net molecular charge or molecular size

Answer 8

chromatography-based extraction method

Question 9

used for cell lysates

Answer 9

chromatography-based extraction method

Question 10

the dna sample is mixed with cesium chloride

Answer 10

etbr-csci gradient centrifugation method

Question 11

how many hours is dna to be ultra-centrifuged?

Answer 11

10 hours

Question 12

acts as intercalation agent

Answer 12

ethidium bromide

Question 13

sample is suspended in an alkaline solution containing NaOH and SDS detegent

Answer 13

alkaline extraction

Question 14

meaning of sds

Answer 14

sodium dodecyl sulfate

Question 15

added to neutralize the solution

Answer 15

potassium acetate

Question 16

selective binding of the negatively charged DNA with the silica
surface covered with positively charged ions

Answer 16

silica extraction

Question 17

used for dna extract

Answer 17

silica extraction

Question 18

The DNA-containing sample is added with lysis buffer, SDS and proteinase K

Answer 18

ctab extraction

Question 19

the initial step involves liberation of DNA from a cell using SDS

Answer 19

phenol-chloroform method

Question 20

main reagent for phenol-chloroform method

Answer 20

phenol-chloroform-isoamyl alcohol

Question 21

used to preserve DNA from being degraded by phenol

Answer 21

chloroform

Question 22

a serine protease from the fungus Engyodontium album

Answer 22

proteinase k

Question 23

can be used for crude cell lysates

Answer 23

salting out method

Question 24

The sample is first mixed with SDS, proteinase K and lysis buffer

Answer 24

silica column-based dna extraction method

Question 25

ideal for automation and require little equipment to perform as it does not depend on centrifugation

Answer 25

magnetic beads

Question 26

Prior of using this, DNA can be washed from the material

Answer 26

cellulose-based paper

Question 27

impregnated with buffers, detergents, and chelating agents that facilitate the extraction of DNA

Answer 27

cellulose-based paper

Question 28

what material is the chelex resin

Answer 28

styrene divinylbenzene

Question 29

material which chelates metal ions that act as cofactors for DNases

Answer 29

chelex resin

Question 30

can be used for tissue samples

Answer 30

chelex-100 extraction

Question 31

appropriate for plant cell samples

Answer 31

filter paper-based dna extraction method

Question 32

disc used in filter paper-bases dna extraction method

Answer 32

whatman filter paper