MBD Laboratory Flashcards
(15 cards)
responsible for the development and perfomance of molecular testing
mbd laboratory
techniques used in mbd lab
pcr, isothermal amplification and sequencing
dna
pcr
rna
rt-pcr
also known as clean room
pre-amplification room
two pre-amplification rooms
reagent and sample preparation room
should be under positive air pressure
pre-amplification rooms
usually one of the cleanest areas in the mbd lab
reagent preparation room
mechanical barriers to prevent contamination
sample preparation room
also known as the dirty room
post-amplification room
dedicated for in vitro amplification analysis
dirty room
also referred as the pcr room
post amplification room
it is where the amplification steps occur
amplification and post-amplification analysis room
instrument used in amplification and post-amplification analysis room
thermocycler
should be under negative air pressure
post-amplification room