DNA Markers I, II, III Flashcards Preview

Biol 351 > DNA Markers I, II, III > Flashcards

Flashcards in DNA Markers I, II, III Deck (24):
1

Adenine binds to

Thymine

2

Guanine binds to

Cytosine

3

Polyelectrolyte theory of the gene says

The negative charges on the two backbones of the DNA molecule spread it out, giving easier access to the machinery that reads and copies the base sequence

4

DNA molecule can be copies by means of ______

DNA polymerization reactions

5

Enzyme responsible for DNA replication

DNA polymerase

6

DNA polymerase adds nucleotides to the ____ end of a polynucleotide strand

3' end

7

Fluorescent molecule used to visualize DNA with UV light

Ethidium bromide

8

Separation of molecules in an electric field, sorted by size

Elecrophoresis

9

Method used to amplify specific sequences of DNA (made in 1984)

Polymerase chain reaction (PCR)

10

A DNA polymerase that can be heated without being distroyed

Taq polymerase

11

A small unit for reaction tubes that can heat up and cool down quickly - used for PCR

A thermal cycler

12

Meiosis is a _________ division

Reductional

13

Maternal inheritance

Via mitochondria

14

DNA polymorphism

a location on a specific chromosome that has appreciable variation

15

SNPs

Single Nucleotide Polymorphism

16

VNTRs

Variable number of tandem repeats

17

STRs

Short tandem repeats

18

Used when a large amount of DNA is availible

VNTR

19

Used when only a small amount of DNA is availible

STR

20

Why are STRs used?

There is no natural selection acting on them

21

How to calculate the probability of a DNA match between two unrelated people

Using the Hardy-Weinberg equilibrium

22

First use of DNA evidence to solve a crime

The Pitchfork Case (1986) in england

23

The first use of DNA evidence to exonerate an innocent person

The Pitchfork Case

24

The first use of a DNA dragnet

The Pitchfork Case