Dna Replication And Enzymes

Question 1

explain DNA polymerases

Answer 1

DNA chain elongation is catalyted by DNA poly merase
DNA polymercases synthesize only from 5’ to 3’
It uses Mg++ ions as cofactor
Replication can accur without it
3’ başlangıca ihtiyacı olduğu için replikasyonu başlatamaz
1-> RNA primary removal and DNA repair
2-> DNA repair
3 and 4 -> chromosom replication
5-> TLS: translation DNA synthesis

Question 2

Discriminator amino acid

Answer 2

check if the suger is ribose or deoxyribose

Question 3

Exonucleases

Answer 3

catalyzing the removal of nucleotidies

Question 4

DNA pol III holoezyme

Answer 4

yanlış bağlanmış hükleotidleri kontrol eder
sliding clamp yerleştirir

Question 5

sliding clamp

Answer 5

DNA polymerazı iter ve sürdürebilirliğini arttırır

Question 6

DNA Pol
Alpha
Beta
Gama
Delta
Epsilon

Answer 6

Alpha: primer synthesis during DNA rep
Beta: Base excision repair
Gamma: mitochondrial DNA rep and repair
Delta: lagging DNA strand
Epsilon: leading strand synthesis

Question 7

replicon

Answer 7

bir bubble’dan diğer bubblea kadar olan mesafe

Question 8

DNA Helicase

Answer 8

replikasyon origini açıldıktan sonra bazlar arasındaki H bağlarını karar
enerji harcar ATP

Question 9

Single-Stran d DNA - Binding Proteins(SSBS)

Answer 9

Helikaz tarafından açılmış tek zincirli DNA’ların birbirine bağlanmasını engeller

Question 10

Primase

Answer 10

DNA sentezinin başlayabilmesi için gerekli dan RNA primelerinin sentezlenmesi

Question 11

Telomerase

Answer 11

Protects DNA by adding sequences to DNA and prevents shortening

Question 12

Telomerase

Answer 12

RNA dependendent DNA polymerase