DNA Technology Flashcards
(6 cards)
1
Q
What is recombinant DNA
A
DNA constructed in the lab from 2 natural / artificial DNA fragments
2
Q
Describe the process of forming recombinant DNA
A
- digestion with appropriate restriction enzymes
- ligated using DNA ligases
- rDNA then cloned via insertion into a vector + amplification into a host cell
3
Q
What is a transgenic host cell
A
A host cell which has been altered by the insertion of recombinant DNA
4
Q
What are 3 uses of DNA cloning
A
- biopharmaceuticals
- gene therapy
- gene analysis
5
Q
What are probes
A
Labelled RNA or DNA fragments with a known sequence used to detect a complementary sequence in a DNA / RNA population
6
Q
A
