DNA Virus 1

Question 1

Where does transcription and replication occur for DNA viruses?

Answer 1

Nucleus

Question 2

What is the origin of the RNA polymerase that transcribes the RNA?

Answer 2

Host

RNA polym 2 and TF recognize viral promoters

Question 3

Does the host or viral DNA polymerase replicate the genome?

Answer 3

Both do

Question 4

Where do the transcription factors come from that regulate RNA polym 2?

Answer 4

Both host and viral TF

Viral TF are important virulence factor

Question 5

Do viruses encode their own DNA polymerases?

Answer 5

Some do and others do not
Some virus genomes are recognized by the host DNA polymerase
Large DNA virus encode their own polymerase and accessory proteins

Question 6

1. Where does diversity come from in DNA viruses?

2. Compare the rate of mutation between DNA and RNA viruses

Answer 6

1. Recombination within and between genomes

2. DNA polymerase has a higher fidelity than RDRP and DNA virus genome is more stable

Question 7

Adenovirus:

1. Diseases
2. Transmission
3. Susceptible population
4. Diagnosis
5. Treatment
6. Prevention

Answer 7

1. Respiratory: bad cold, fever, laryngitis and cough, pneumonia, acute hemorrhagic cystitis, Epidemic keratoconjunctivitis, Gastroenteritis
2. Aerosol, fecal-oral, objects, poorly chlorinated pool
3. Children and military
4. Antigenic detection, PCR, Serology
5. cidofovir only for severe cases because it is nephrotoxic
6. vaccine only for military

Question 8

Describe the Attachment, entry and uncoating of adenovirus life cycle

Answer 8

Fiber proteins bind cell receptor
Enter by endocytosis
endosome acidification cause fibers to lyse cell
Caspid moves to nucleus and DNA uncoats through nuclear pore

Question 9

Describe Adenovirus life cycle

Answer 9

genome replicates in nucleus
HOST RNA Pol 2 makes mRNA
Gene expression: Immediate Early, Early, Late
Genome replication by viral DNA pol
Caspid assembly in nucleus
Egress by lysis

Question 10

HPV

1. Disease
2. Diagnosis
3. Treatment
4. Prevention

Answer 10

1. Epithelial Disease: Common, plantar, and genital warts
   Malignancies: Head and neck, Cervical and penile cancers
2. DNA tests for HPV type
3. freezing, salicylic acid, surgery for warts
   oncotherapy for cancers
4. Gardasil and cervarix (VLPs = empty caspids)

Question 11

Explain how HPV starts is life cycle and the possible results from that location

Answer 11

Virus moves all the way through skin until the basal epithelial. As the skin layers shed and moves outward so does the virus. Along the way calls are transformed into virial cells in the skin layers.
Alternatively it can move the opposite way ( into the dermis) and form a melignancy

Question 12

HPV DNA replication features

Answer 12

Host RNA Pol transcribes and Host DNA Pol synthesizes viral genome

Question 13

What are viral factors E6 and E7

Answer 13

oncogenes