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Flashcards in Electrophysiology Deck (40):

What are the types of action potential present in the heart? Are these fixed?

Working myocardium: with a plateau , and stable resting membrane potential. Occurs in atrial and ventricular myocytes. Pacemaker tissue: no stable membrane potential. In AV, SA nodes. **as you move between the two the action potential types blend/transition to the other. Purkinje fibers and bundle of His are a blend ***


Differences between cardiac muscle and skeletal muscle action potentials

- cardiomyocyte AP is much longer and lasts the length of the contraction so it can't go into tetanus - cardiomyocyte (working, not pacemaker ) resting membrane potential more negative (closer to equilibrium potential for potassium)


If AV node, SA node, bundle of His and purkinje fibers all have intrinsic pacemaker activity, why is only the SA node the pacemaker?

Because the SA node has the fastest repolarization because it has the most "funny current" channels. So it's impulse will reach the other areas before they have a chance to fire.


EC coupling in cardiomyocytes

1)action potential reaches dyad/triad2) voltage gated Ca channels open3) Ca binds to Ryr2 and causes Ca release from calsequestrin in SR (Ca channel and Ryr2 NOT physically coupled)4) Ca --> troponin-->tropomyosin-->myosin binding-->contraction4) Ca is resequestered by SERCA or extruded by Na/Ca exchanger


What are the three states of voltage gated sodium channels, calcium channels and transient outward potassium channels and which order must they proceed in? What variable does each transition depend on?

Open--> Inactivated --> Closed Open--> Inactivated depends on time Inactivated----> Closed depends on membrane potential


Phases of a working cardiomyocyte action potential

0- fast depolarization. 1- repolarization with Na channels inactivated and k channels open 2- plateau with inward Ca balancing outward potassium 3- repolarization, pretty rapid 4- resting membrane potential


Mechanical effects of B1-adrenergic stimulation

-positive inotropy (force of contraction increases)-positive lusitropy (rate of relaxation increases)


Channels and currents involved in phase 0 of a working cardiomyocyte action potential

- voltage gated sodium channels open, rapidly depolarize the cell then inactivate - inward sodium current attempts to reach the Nernst equilibrium potential for sodium.


Channels and currents involved in phase 1 of a working cardiomyocyte action potential

- sodium channels are inactive - potassium channels carrying the transient outward current Ito are open


Molecular mechanisms of positive inotropic and positive lusitropic effects

All mediated by PKA phosphorylation of key proteins.Inotropy-more Ca enters cell with each action potential-SERCA increases velocity-therefore more Ca released by Ryr2Lusitropy-troponin C has less affinity for Ca...lets go sooner-increased SERCA velocity


Channels and currents involved in phase 2 of a working cardiomyocyte action potential

- slow inward ( Isi) AKA Ica, carry calcium into the cell and this balances the outward potassium current ( Ito) at the beginning, and then rapid ( Ikr) and slow (Iks) potassium outward currents (these are called delayed rectifiers)


Channels and currents involved in phase 3 of a working cardiomyocyte action potential

- Calcium channels are inactive - outward potassium current is carried by delayed rectifiers


Channels and currents involved in phase 4 of a working cardiomyocyte action potential

-inward rectifier potassium current directs an outward current of potassium


Channels and currents involved in phase 0 of a pacemaker action potential

-depolarization mediated by slow inward calcium current ( Isi AKA Ica) - No sodium channels


Phases of the action potential in a pacemaker cell

0- depolarization mediated by slow inward calcium current 3-repolarization, funny current starts 4- diastolic depolarization. Analogous to resting membrane potential in cardiomyocytes


Channels and currents involved in phase 3 of a pacemaker action potential

- repolarization due to rapid ( Ikr) and slow ( Isi) delayed potassium rectifiers -The funny current ( both Na and K along their gradients) begins and intensifies and the membrane potential gets more negative


Channels and currents involved in phase 1 of a pacemaker action potential

- the funny current lets sodium in and potassium out - this slowly depolarizes the membrane until threshold is reached


Mechanism behind absolute and relative refractory period in cardiomyocytes

Absolute: sodium channels are inactive and will not convert to a closed state until a certain, negative membrane potential is reached. Relative: a proportion of the channels have become closed, and are now free to open again


Factors influencing conduction velocity

1) Size of cells: smaller cells resist flow 2) Number of gap junctions: more is faster 3) Rise of action potential:


Cardiac cells ranked from fastest to slowest conduction

Purkinje cells > Bundle of His > ventricle/atria > SA and AV nodes


The relative number of beats per minute of pacemaker cells in the heart

SA > AV > bundle of His/ Purkinje fibers


Components of the atrial conducting system

-SA node ( posterior RA wall) - Bachman's bundle (RA-->LA) - Intranodal branches (SA node-->AV node)


Components of the ventricular conduction system

- AV node ( inferior, medial part of RA) - bundle of His ( from AV node into interventricular septum) - right and left bundle branches (RBB, LBB) ( run down septum parallel) - purkinje fibers ( come off right and left branches the whole way down)


Location and function of the cardiac skeleton

Between atria and ventricles, fibrous tissue that does not conduct an action potential. Its function is to make the action potential go through the AV node and through the bundle of His to get into the ventricles.


What kind of tissue is the cardiac conduction system



How does depolarization spread through the heart

Through the fast conducting fibers, and then out into the myocardium via gap junctions.


Bipolar limb leads

Lead 1: RA (-) and LA (+) Lead 2: RA(-) and LL (+) Lead 3: LA (-) and LL (+)

A image thumb

Unipolar limb leads

a= augmented, V = voltage, f= foot aVR: RA(+) and LL, LA (-) aVL: LA(+) and RA, LL (-) aVF: LL(+) and RA, LA (-) ** the one in the name ( R or L or F) is always positive while the other two are negative**

A image thumb

Limb leads measure electrical activity in which plane?

Frontal ( coronal)


Precordial leads measure electrical activity in which plane?

Horizontal ( transverse)


Precordial recording electrodes are always....



When do you get an upwards deflection on an ECG?

When depolarization moves towards a positive electrode OR repolarization moves away from a recording electrode. AKA a positive deflection


When do you get a downwards deflection on an ECG?

When depolarization moves away from the positive electrode


What is the flat line on and ECG called?

Isoelectric line


What do P, QRS , and T represent?

P: atrial depolarization QRS: ventricular depolarization T: ventricular repolarization


Why is the T wave a positive deflection?

The last cells to depolarize are the first cells to repolarize. So the wave of repolarization moves from epicardium to endocardium. A repolarization moving away from a positive electrode is the same thing as a depolarization moving towards an electrode.


Histological differences between skeletal and cardiac muscle

- cardiac has LOTS of mitochondria (O2 dependence)-cardiac has smaller SR-cardiac has dyads and triads where EC coupling happens



-inotropy is the force of contraction-Lusitropy is how quickly the heart relaxes-chronotropy is how fast the heart relaxes


What would happen if we didn't have positive lusitropy when our heart rate increased

- We'd spend less time in diastole, which would mean less filling and less CO-Coronary circulation would decrease because it only works during diastole.


Energy utilisation of cardiomyocytes (at "rest" and during a burst of exercise

70% fatty acid oxidation20% glycolysis10% lactateexcept during burst of exercise when creatine phosphate and glycolysis become main sources for a little bit. BUT glycolysis alone cannot support the demands of cardiomyocytes