ELISA Flashcards
1
Q
ELISA stands for?
A
Enzyme-linked immunosorbent assay
2
Q
What kind of ELISA do we use?
A
sandwich
3
Q
ELISA 1st step
A
Capture antibodies are coated on plate
4
Q
ELISA 2nd step
A
Target Antigen (our sample, AAV serotype) attaches to antibody
5
Q
ELISA 3rd step
A
Detection antibody carrying Horse Radish Peroxidase (or alkaline phosphatase)
6
Q
ELISA 4th step
A
Substrate (TMB or ABTS)
7
Q
ELISA 5th step
A
HRP catalyzes substrate which yields a colored product
8
Q
What is the purpose of ELISA?
A
protein quantification
