TCID50 Flashcards
1
Q
TCID50 Stands for
A
Tissue Culture Infectious Dose 50%
2
Q
What is TCID50 used for
A
determine infectivity of virus and determine dosing of sample
3
Q
Why use RC32 HeLa cells for TCID50?
A
RC32 HeLa cells contain the rep and cap gene missing in sample
4
Q
CPE stands for
A
cytopathic effect
5
Q
What is CPE?
A
CPE is observed when cells are inflated / filled with virus
6
Q
Why serially dilute for TCID50?
A
Serially dilute to find 50% of cells that are infected with virus
7
Q
Karber Analysis needs
A
Where 100% and less than 50% of cells are infected and calculates TCID50/mL through log calculations
