SDS-PAGE Flashcards
1
Q
What does SDS-PAGE stand for?
A
Sodium dodecyl sulfate (sodium lauryl sulfate) – polyacrylamide gel
2
Q
What is the purpose of SDS-PAGE?
A
protein purification
3
Q
What is SDS used for?
A
detergent with strong protein-denaturing effect and binds to protein backbone
4
Q
What is reducing agent used for?
A
cleaves disulfide bonds preventing folding, proteins unfold into linear chains with negative charge
5
Q
How are proteins separated?
A
based on chain length by electrophoresis
6
Q
Which chain length moves faster through electrophoresis?
A
Smaller chains move faster than longer chains
