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ENTEROBACTERIACEAE Flashcards

(55 cards)

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1
Q

Enterobacteriaceae is a
Gram ___ bacilli
___ anaerobe
___ spore former

A

negative
facultative
non

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2
Q

Enterobacteriaceae is an
Oxidase ___ EXCEPT P. ____
Catalase ___ EXCEPT ____
Xylose fermenters EXCEPT ____
Motile EXCEPT ____, ____, Y. ____
Non-encapsulated EXCEPT ____ & ____
Aerogenic EXCEPT S. ____, ____, ____, ____

A

-; P. shigelloides
+; Shigella (S. dysenteriae)
Shigella
Shigella, Klebsiella, Y. pestis
Klebsiella & Enterobacter
S. typhi, Yersinia, Shigella, Providencia

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3
Q

T/F
Enterobacteriaceae ferments glucose

A

TRUE

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4
Q

TRUE PATHOGENS (3)

A
  1. Lactose Fermenters
  2. Non-Lactose Fermenters
  3. Late Lactose Fermenters
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5
Q

Lactose Fermenters (EKE)

A

Escherichia, Klebsiella, Enterobacter

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6
Q

T/F
Klebsiella and Enterobacter are siblings

A

TRUE

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7
Q

Non-lactose Fermenters

A

All enterobacteriaceae family

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8
Q

Late lactose Fermenters (CAE)

A

Citrobacter, Arizonae, Y. Enterocolitica

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9
Q

ANTIGENS
- K antigen - heat ____
- O antigen - heat ____
- H antigen - heat ____

A

labile
stable
labile

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10
Q
  • 1st genus
  • Common name is Colon bacillus
A

E. coli

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11
Q

E. coli is a normal flora of

A

GIT

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12
Q

E. coli
Predominant ____ anaerobe in ____ and have ____ antigens
Primary virulence factor: ____

A

facultative; Large intestine; 3 (KOH)
PILI

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13
Q

E. coli on BAP

A

BETA hemolytic- Clear of zone hemolysis

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14
Q

T/F
E. coli is mostly motile

A

TRUE

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15
Q
  • # 1 cause of community-acquired UTI
  • # 1 gram-negative SEPSIS
  • # 2 NEONATAL meningitis-nosocomial, BACTEREMIA
A

E. coli

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16
Q

E. coli is ____ in water - fecally contaminated

A

positive

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17
Q

E. coli Serotypes (5)

A
  1. Enteropathogenic E. coli or EPEC
  2. Enteroinvasive E. coli or EIEC
  3. Enteroaggregative E. coli or EAEC
  4. Enterotoxigenic or ETEC
  5. Enterohemorrhagic or EHEC
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18
Q

non bloody diarrhea (no blood, no mucus and no toxins; non invasive, non into toxigenic)

A

EPEC Infantile diarrhea

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19
Q

EPEC
Virulence factor: ____

A

Pili - pathogenicity of islands

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20
Q

Lesions called “attaching and effacing lesions

A

Enteropathogenic E. coli or EPEC

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21
Q

produce dysentery-like diarrhea (watery to bloody diarrhea with RBC, mucus and neutrophils)

A

EIEC Shigella-like disease

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22
Q

EIEC
Virulence factor:
Test:

A

Invasin
Sereny test

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23
Q

EAEC 2 types

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A
  1. Diffusely adherent E. coli (DAEC)
  2. Enteroaggregative E. coli (EAEC)
24
Q

Type of EAEC that causes UTI (chronic/recurring) and diarrhea

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A

Diffusely adherent E. coli (DAEC)

25
Type of EAEC that causes “stacked-brick pattern” (no blood but persistent diarrhea) located in cells or between the cells by fimbriae or pili
Enteroaggregative E. coli (EAEC)
26
EAEC Virulence Factor:
Aggregative regulator gene (AggR gene)
27
Traveller’s diarrhea Causes Hemorrhagic colitis Produces Watery diarrhea
Enterotoxigenic or ETEC
28
- It has a perfused watery stool - most common diarrhea - self-limiting, non invasive, and no blood and WBC
ETEC Montezuma’s revenge
29
presence of colonization factor antigen which produces heat labile and heat stable enterotoxins
ETEC Adhesion
30
Grossly bloody diarrhea Shigella-like toxin
Enterohemorrhagic or EHEC
31
EHEC causative agent
E.coli 0157:H7
32
- this produces shigella-like toxins- which is life-threatening (verotoxin), associated with undercooked infected hamburger - Colorless - (-) in MUG test
EHEC
33
EHEC Toxins (2)
1. Verotoxins 1 (VT1) 2. Verotoxins 2 (VT2)
34
EHEC Toxin that is identical to shigella dysenteriae Type 1 (Shiga Toxin)
Verotoxins 1 (VT1)
35
EHEC Toxin that is similar biologically but immunologically different to VT1
Verotoxins 2 (VT2)
36
- most severe manifestation of EHEC - undercooked hamburger, dairy products and apple cider
EHEC HUS - hemolytic Uremic Syndrome
37
In EHEC it replaces lactose to sorbitol
SMAC - Sorbitol Macconkey Agar
38
Extraintestinal E.coli strains (UM)
1. Uropathogenic E. coli (UPEC) 2. Meningitis/sepsis-associated E. coli (MNEC)
39
Extraintestinal E.coli strains that cause cystitis and acute pyelonephritis
Uropathogenic E. coli (UPEC)
40
Extraintestinal E.coli strains that cause neonatal meningitis
Meningitis/sepsis-associated E. coli (MNEC)
41
- 4-methylumbelliferyl-beta-D-glucuronide E. coli broth; - (+) result is Electric Blue fluorescence
MUG Test
42
Klebsiella spp are:
- Lactose Fermenters - Encapsulated - Non motile
43
Klebsiella Mucoid: - ___ is pink and dry - ___ and ____ are both pink but not darker as E. coli
E. coli Klebsiella and Enterobacter
44
Klebsiella Virulence factor: Most resistant antibiotic:
Capsule Ampicillin
45
Ventilator-associated pneumonia, wound infections, UTIs
Klebsiella
46
- Common Name: Fried landers bacillus - 2nd cause of UTI - Produce secondary pneumonia
K. pneumoniae
47
- classic pneumonia - emit bloody sputum - associated with community acquired pneumonia that produces a currant jelly like sputum
K. pneumoniae
48
K. pneumoniae distinct feature
large polysaccharide capsule
49
- ESBL- Extended-Spectrum Beta-lactamases - (+) in Neufeld Quellung
K. pneumoniae
50
T/F In K. pneumoniae, Neufeld Quellung test is used if a bacteria has nucleus
FALSE Neufeld Quellung test is used if a bacteria has CAPSULE
51
- a disease in nasal mucosa, biochemically inactive - Atrophic rhinitis - Plasmid-mediated ESBLs
K. pneumoniae subsp. ozaenae
52
- Donovanosis - STD - Safety pin appearance - Requires additional nutrients for growth
K. granulomatis
53
K. granulomatis diagnositc feature
DONOVAN body/BEEFY RED ULCER (blue stain with prominent granules); intracellular microorganisms
54
- Disease similar to K. pneumoniae - indole (+)
K. oxytoca
55
- Raoultella planticola - which is an environmental microorganism
K. planticola

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