ENTEROBACTERIACEAE PT.2

Question 1

this genus causes dysentry

Answer 1

Shigella

Question 2

biochem inert of shigella

Answer 2

Non-lactose fermenters
Do not use acetate or mucate as carbon source

Question 3

unique member of shigella

Answer 3

shigella sonnei

Question 4

specie that is fragile and susceptible to
various effects of physical and chemical
agents

Answer 4

Shigella species

Question 5

why shigella sonnei is unique?

Answer 5

Ability to ferment lactose slowly (LLF) producing pink colonies on MAC agar only after 8 hours of incubation

ONPG positive

Question 5

t/f: Shigella are susceptible to the acid pH of
the stool.

Answer 5

t

Question 6

what happens if k antigens are present

Answer 6

it interferes with o antigen in serologic grouping

Question 6

what must be done in order to remove k antigen

Answer 6

boil the bacterial suspension for 30 minutes

Question 6

shigella subgroup b (strong’s bacillus)

Answer 6

shigella flexneri

Question 6

shigella subgroup a (dysentry bacillus)

Answer 6

shigella dysentriae

Question 6

shigella subgroup c (shigella ambigua)

Answer 6

shigella boydii

Question 7

shigella subgroup d

Answer 7

shigella sonnei

Question 7

shigella specie that is predominant in the U.S and other industrialized countries

Answer 7

shigella sonnei

Question 7

shigella species that are the most common isolates in developing countries.

Answer 7

shigella dysentriae type 1 and shigella boydii

Question 8

reservoir of shigella

Answer 8

humans and large primates

Question 8

mot of shigella

Answer 8

direct person-to-person contact
fecal-oral route
flies, fingers, food and water contaminated by infected persons

Question 8

shigella specie that affects mostly young children. Leading isolate of gastroenteritis among men who have sex with men. HIV infections increases this risk.

Answer 8

shigella flexneri

Question 9

shigella specie that remains the most virulence of all the species with a significantly high morbidity and mortality rate.

Answer 9

shigella dysentriae type 1

Question 10

function of cell-to-cell passage

Answer 10

protects Shigella from immune-mediated clearance

Question 10

enrichment medium for the isolation of Salmonella spp., and Shigella species from heavily contaminated specimens such as stool.

Answer 10

Selenite broth

Question 10

function of IL1B

Answer 10

acts as a chemoattractant for PMNs into the infected tissues.

destabilizes the integrity of the intestinal wall and allows bacteria to reach the deeper epithelial cells.

Question 11

what is the result if there are high contrentations of gb3

Answer 11

hemmorhagic diarrhea and hemolytic uremic syndrome

Question 11

typically found in cold-blooded animals as well as in rodents and in birds, which serve as their natural hosts.

Answer 11

salmonella

Question 11

H Ag

Answer 11

More stable
O and H are the primary antigenic structures

Question 11

Fimbriae

Answer 11

for adhesion

Question 11

O Ag

Answer 11

Stable to boiling and acid alcohol

Question 12

Vi Ag

Answer 12

Plays a role in preventing phagocytosis

Question 12

also known as the Gardner’s bacillus

Answer 12

salmonella enteritidis

Question 12

also known as the Eberth’s bacillus

Answer 12

salmonella typhi

Question 12

what is rose-spots

Answer 12

blanching rose-colored papules around the umbilical region also appear during the second week of fever,

Question 12

diseases associated with salmonella

Answer 12

gastroenteritis or food poisoning typhoid fever

Question 12

most severe form of enteric fever

Answer 12

Typhoid fever

Question 12

initially isolated from a lizard and is usually isolated from cold-blooded animals and the environment.

Answer 12

salmonella bongori

Question 12

salmonella spp that have no known animal reservoirs and infections only occurs in humans.

Answer 12

salmonella typhi salmonella paratyphi

Question 12

other species that causes enteric fever

Answer 12

Salmonella Paratyphi A, B,and C salmonella cholerasius

Question 12

during the first week of enteric fever where is the bacteria isolated?

Answer 12

isolated from blood

Question 13

can be used indefinitely as clinical specimen

Answer 13

Stool

Question 13

during the second and thrid week of enteric fever where is the bacteria isolated?

Answer 13

isolated from the stool

Question 13

colony characteristic in SSA

Answer 13

colorless-black center (H2S+)

Question 13

colony characteristic in EMB/MAC

Answer 13

colorless

Question 13

colony characteristic in NA

Answer 13

maple leaf colonies w/irregular margins

Question 13

colony characteristic in XLD

Answer 13

slightly pink to white opaque colonies

Question 13

qualitative in widal test

Answer 13

slide technique

Question 14

Serologic test that detects the presence of antibodies in the person’s serum against O and H antigens of Salmonella Typhi.

Answer 14

Widal Test

Question 14

what type of colony does salmonella typhi on bsa produce

Answer 14

Metallic colonies with a black ring

Question 15

titer of o agglutinins that is clinically significant as these indicate active infection.

Answer 15

1:100

Question 16

quantitative in widal test

Answer 16

tube technique

Question 17

titer of h agglutinins that is clinically significant as these indicate active infection.

Answer 17

1:200

Question 17

pathogens under yersinia

Answer 17

Yersinia pestis Yersinia pseudotuberculosis Yersinia enterocolitica

Question 18

causative agent of plague

Answer 18

Yersinia pestis

Question 19

Implicated in sporadic cases of gastroenteritis, mesenteric lymphadenitis especially in children, and generalized septicemic infections in immunocompromised hosts.

Answer 19

Yersinia pseudotuberculosis Yersinia enterocolitica

Question 20

has been a cause of diarrhea outbreaks in numerous communities and it has been seen to mimic appendicitis.

Answer 20

Yersinia enterocolitica

Question 21

The causative agent of plague – a primary disease in rodents that are spread to humans by bites of fleas.

Answer 21

Yersinia pestis

Question 22

Parasite of the rodent of yersinia pestis

Answer 22

Rattus rattus

Question 23

flea vector (most common and effective vector of Yersinia pestis)

Answer 23

Xenopsylla cheopis

Question 24

stain used in cells to have a characteristic “Safety Pin” appearance

Answer 24

Wayson Stain

Question 25

most common form of the disease, results from the bite of a flea, or by direct inoculation of an open skin would by plague-infected material.

Answer 25

Bubonic plague

Question 25

results from the hematogenous dissemination of the organism, following the damaged of the lymph nodes in patients with bubonic plague.

Answer 25

Secondary septicemic plague

Question 26

occurs in patients without buboes as primary infections.

Answer 26

Primary septicemic plague

Question 27

results form the dissemination of the organism to other organ systems such as the lungs, brain, liver, and spleen, with daysnear and hemoptysis, plague meningitis, and hepatic and splenic abscesses.

Answer 27

Secondary pneumonic plague

Question 28

yersinia specie that is found in a wide variety of animals, including domestic swine, cats, and dogs.

Answer 28

Yersinia enterocolitica

Question 29

mot of yersinia enterocolitica

Answer 29

contact with household pets

Question 30

used to increase the recovery in fecal samples.

Answer 30

Cold-enrichment

Question 30

Clinical manifestations of Yersinia enterocolitica

Answer 30

§ Acute gastroenteritis § Appendicitis – like syndrome § Less frequent manifestations such as septicemia, arthritis, and erythema nodosum.

Question 31

yersinia specie that has the ability to survive in cold temperatures

Answer 31

Yersinia enterocolitica

Question 32

Similar to Yersinia pestis for being a disease primarily of rodents, particularly guinea pigs. And birds are natural reservoirs.

Answer 32

Yersinia pseudotuberculosis