Enterobacteriaciae Flashcards
1
Q
Most are present in the intestinal tract as commensal flora except
A
Salmonella
Shigella
Yersinia
2
Q
All members are motile except
A
SKY
Shigella
Kingella
Yersinia
3
Q
All are nonencapsulated except
A
Klebsiella
Enterobacter
4
Q
All members ferment ____ and reduce nitrate to nitrite
A
Glucose
5
Q
Only oxidase + member of the enterobacteriaciae
A
P. shigelloides
6
Q
ESBL-Producing bacteria
A
E. coli
K. pneumoniae
K. oxytoca
7
Q
Isolated from urinary tract and cause bacteremia
A
E. coli
K. pneumoniae
P. mirabilis
8
Q
Antibiotic-resistant genera
A
CES
Citrobacter
Enterobacter
Serratia
9
Q
Associated with diarrhea
A
YESS
Yersinia
E. coli
Shigella
Salmonella
10
Q
Heat stable antigen
A
Somatic “O” Ag
11
Q
Antigen located on the cell wall for E. coli and Shigella serotyping
A
Somatic “O” Ag
12
Q
Somatic “O” Ag
O stands for
A
Ohne
13
Q
Heat labile antigen found in the flagellum for Salmonella serotyping
A
Flagellar “H” Ag
14
Q
Flagellar “H” Ag
H stands for
A
Hauch
15
Q
Heat labile polysaccharide; covers the O Ag; found as K1 Ag of E. coli and Vi antigen of S. enterica subsp. enterica serotype typhi
A
Capsular “K” Ag
16
Q
Capsular “K” Ag
K stands for
A
Kapsel
17
Q
Common name of E. coli
A
Colon Bacillus
18
Q
Primary marker of fecal contamination in water purification
A
E. coli
19
Q
Leading cause of nosocomial infection- UTI
A
E. coli
20
Q
E. coli “O” groups have shown cross reactivity with the “O” Ag of _____
A
Shigella
21
Q
Appearance of E. coli in EMB
A
Greenish metallic sheen
22
Q
Virulence factor of E. coli that is similar to the Vi Ag of Salmonella
A
K1 Ag
23
Q
Causes neonatal meningitis
A
E. coli
24
Q
Formerly called E. coli atypical
A
E. hermanii
25
CA of Infantile Diarrhea
EPEC
Enteropathogenic E. coli
26
Causes diarrhea with mucus but w/o blood
EPEC
Enteropathogenic E. coli
27
(+) H Ag and Intimin
EPEC
Enteropathogenic E. coli
28
E. coli strain which causes loss of micro villi but no toxin production
EPEC
Enteropathogenic E. coli
29
CA of Traveler's Diarrhea/Montezuma's Revenge
ETEC
Enterotoxigenic E. coli
30
Virulence factors: Heat-stable and Heat Labile enterotoxins
ETEC
31
Persons with Achlorhydria are at high risk
ETEC
32
CA of Dysentery-like/Shigella-like infection
EIEC
33
CA of watery diarrhea with WBCs
EIEC
34
Virulence factor of EIEC
Invasin
35
Virulence test for Shigella and EIEC
Sereny Test
36
CA of Hemorrhagic colitis, HUS, and TTP
EHEC
Enterohemorrhagic E. coli
(E. coli O157:H7)
37
Virulence factors of EHEC
Cytotoxins (Verotoxin I and II)
38
Only E. coli strain that is MUG (-)
EHEC
39
E. coli strain that is (+) Verotoxin/Shiga-like toxin
EHEC
40
CA of bloody diarrhea without WBCs
EHEC
41
E. coli strain with "stacked-brick" appearance
Enteroadherent E. coli (EAEC) or Enteroaggregative E. coli
42
4-methylumbelideryl B-D glucoronide is for what test
MUG
43
Determines the organism's ability to produce keratoconjunctivitis in guinea pig
Sereny Test
44
E. coli strain that appears colorless on Mac Conkey Agar and produce heavy growth
E. coli O157:H7
45
E. coli strain that rarely produces B-glucoronidase resulting to MUG test negative
E. coli O157:H7
46
E. coli strains that produce verotoxins are also called
Shiga toxigenic E. coli (STEC)
47
If ETEC is suspected, a ____ should be inoculated as well because it only grows on this plate
BAP
48
Associated with Choler-like toxin or heat labile enterotoxin
ETEC
49
E. coli strain that causes gastroenteritis in children
EPEC
50
E. coli that is non lactose, anaerogenic, and non-motile
Alkalescens dispar
51
Test to detect bacterial endotoxin
Limulus Test
52
Limulus in limulus test is derived from
Limulus horse crab
53
Differentiate E. coli from Shigella based on
MacConkey
Acetate
Motility
E. coli Shigella
LF NLF
+ -
+ -
54
Common name of Klebsiella pneumoniae
Friedlander's bacillus
55
Klebsiella pneumoniae is transmitted through droplets
True or False
FALSE
56
Klebsiella pneumoniae string test result
Positive due to capsule
57
Klebsiella spp that are indole positive
indOle POsitive
1. K. oxytoca
2. K. ornithinolytica
58
Klebsiella pneumoniae subsp. pneumoniae is formerly known as
Bacillus capsulatus
59
CA of community acquired pneumonia
K. pneumoniae
60
Virulence factor of K. pneumoniae
Polysaccharide capsule
61
K. pneumoniae
Growth on media with KCN:
Positive
62
Potassium cyanide inhibits
Bacterial growth
63
Characteristic sputum in community acquired pneumonia
Currant jelly-like sputum
64
Klebsiella spp that is biochemically inert
Negative to: LDC, VP and Urease, and Indole
K. rhinoscleromatis
65
Enterobacter resembles ____ on MacConkey agar
Klebsiella
66
Common isolate of Enterobacter
E. cloacae
67
All enterobacter are urease negative except
E. gergoviae
68
Enterobacter that is LDC (-) control
E. cloacae
69
Enterobacter that is Arginine Dihydrolase (+) (2)
1. Enterobacter cloacae
| 2. Enterobacter sakazakii
70
LDC (+) control
K. pneumoniae
71
LDC (+) organisms (7)
KEEESSer sa Arizona
Klebsiella Salmonella
Enterobacter. Serratia
E. coli. Arizona
Edwardsiella
72
A pathogen in neonates causing meningitis and bacteremia, often coming from powdered infant formula
Cronobacter sakazakii
| Formerly known as Enterobacter sakazakii
73
Enterobacter that have a characteristic yellow-pigmented colonies
Cronobacter sakazakii
74
Formerly known as Enterobacter agglomerans
Pantoea agglomerans
75
CA of septicemia due to contaminated IV fluids
Pantoea agglomerans
76
Triple decarboxylase negative
Pantoea agglomerans
77
Serratia are DNAse ____
+
78
Contaminant in antiseptic solutions used for joint infections causing an epidemic of septic arthritis
Serratia marcescens
79
Serratia that causes osteomyelitis following a motorcycle accident
Serratia plymuthica
80
Pigment responsible for pink or red color production by Serratia
Prodigiosin
81
Serratia that produces musty-punget odor or "potato-like" odor
S. odorifera
82
Pigment production of Serratia is well-observed at what temperature
RT (25 C)
83
Serratia that causes blood bag contamination
S. liquifaciens
84
Can cause AGN
Proteus
85
Rapid urease producer
Proteus
86
Common isolate of Proteus
P. mirabilis
87
Odor produce by Proteus
"Burnt chocolate" or "burnt-gun powder" odor
88
Culture appearance of Proteus
Swaming phenomenon
89
Also known as spreading or production of wave-like patterns by Proteus
Swarming
90
#2 Cause of UTI
Proteus
91
Positive Dienes Phenomenon
P. mirabilis
92
Indole positive Proteus
P. vulgaris
93
Same biochemical reaction with P. vulgaris except citrate negative
Morganella
94
Has been isolated from cold-blooded and warm blooded animals
Edwardsiella
95
Human pathogen Edwardsiella
E. tarda
96
Produces colonies on Mac Conkey agar that resembles E. coli and biochemically resembling Salmonella
Citrobacter
97
Slow urease producers
Citrobacter
98
Differentiate Citrobacter from Salmonella based on:
Urease
LDC
Mac Conkey
Citrobacter Salmonella
+ -
- +
LF NLF
99
All Salmonella are NLF except
Arizona
100
Indole negative citrobacter
C. freundii
101
CA of Typhoid Fever
Salmonella
102
Typhidot biologic false +
Dengue Fever
103
Characteristic stool of salmonellosis
Pea soup stool
104
"fish eye colonies"
Salmonella
105
Appearance of Salmonella in HE, BSA, XLD
Colonies with black center
106
Appearance of Salmonella in SSA
Colorless colonies with black centers
107
The one who spread Salmonella
Typhoid Mary
108
Also known as Typhoid Fever
Enteric Fever
109
The organ that is site of long-term carriage of S. serotype typhi
Gallbladder
110
Characteristic of enteric fever that appears during the 2nd week of fever
Rose spots
111
Specimens for Salmonella Identification
1st week of infection
2nd week
3rd week
Blood
Stool
Urine
112
Confirmatory Test for Typhoid Fever
Bone marrow culture
113
Can cause AGN
Proteus
114
Rapid urease producer
Proteus
115
Common isolate of Proteus
P. mirabilis
116
Odor produce by Proteus
"Burnt chocolate" or "burnt-gun powder" odor
117
Culture appearance of Proteus
Swaming phenomenon
118
Also known as spreading or production of wave-like patterns by Proteus
Swarming
119
#2 Cause of UTI
Proteus
120
Positive Dienes Phenomenon
P. mirabilis
121
Indole positive Proteus
P. vulgaris
122
Same biochemical reaction with P. vulgaris except citrate negative
Morganella
123
Has been isolated from cold-blooded and warm blooded animals
Edwardsiella
124
Human pathogen Edwardsiella
E. tarda
125
Produces colonies on Mac Conkey agar that resembles E. coli and biochemically resembling Salmonella
Citrobacter
126
Slow urease producers
Citorbacter
127
Differentiate Citrobacter from Salmonella based on:
Urease
LDC
Mac Conkey
Citrobacter Salmonella
+ -
- +
LF NLF
128
All Salmonella are NLF except
Arizona
129
Indole negative citrobacter
C. freundii
130
CA of Typhoid Fever
Salmonella
131
Typhidot biologic false +
Dengue Fever
132
Characteristic stool of salmonellosis
Pea soup stool
133
"fish eye colonies"
Salmonella
134
Appearance of Salmonella in HE, BSA, XLD
Colonies with black center
135
Appearance of Salmonella in SSA
Colorless colonies with black centers
136
The one who spread Salmonella
Typhoid Mary
137
Also known as Typhoid Fever
Enteric Fever
138
The organ that is site of long-term carriage of S. serotype typhi
Gallbladder
139
Characteristic of enteric fever that appears during the 2nd week of fever
Rose spots
140
Specimens for Salmonella Identification
1st week of infection
2nd week
3rd week
Blood
Stool
Urine
141
Confirmatory Test for Typhoid Fever
Bone marrow culture
142
Shigella is transmitted by Four F's which stand for
Flies
Fingers
Food
Feces
143
Virulence factor of Shigella
Shiga toxin
144
Most virulent shigella spp
S. dysenteriae
145
Shigella that causes Gay Bowel Syndrome
S. flexneri
146
Sensitive to pH changes. Thus, stool should be processed immediately to recover this organism
Shigella
147
Unique in its ability to decarboxylate ornithine among the Shigella spp. (LLF) (ONPG+)
Shigella sonnei
148
Highly communicable due to low infective dose required to produce the disease
Bacillary dysentery
149
Most common cause of communicable diarrhea
S. dystenteriae
150
Shigella sonnei cross react with
Pleisiomonas shigelloides
151
Causative agent of enterocolitis - waterborne gastroenteritis
Yersinia enterocolitica
152
Motile at 22 but not at 35 C
Yersinia enterocolitica
153
Requires cold enrichment technique using phosphate buffered saline for several weeks for isolation from fecal material
Yersinia enterocolitica
154
Has been isolated from contaminated packed RBCs
Y. enterocolitica
155
"bull's eye colonies" in CIN
Y. enterocolitica
Same with Aeromonas (To diff. - Oxidase Test)
Aeromonas +
Yersinia -
156
CA of appendicitis and blood bag contamination
Y. enterocolitica
157
Can be acquired by drinking unpasteurized milk and consumption of incompletely cooked food
Y. enterocolitica
158
Plague Bacillus
Y. pestis
159
Class A Bioterrorism Agent
Y. pestis
160
The only enterobacteriaciae that is transmitted to humans by the bute of an infected flea - Xenopsylla cheopis
Y. pestis
161
CA of bubonic plague - "black death or 6th century pandemic"
Y. pestis
162
Bipolar staining or closed safety pin appearance in microscope using Wayson or Methylene Blue Stain
Y. pestis
163
Appearance of "Salactite Pattern" in Broth
Y. pestis
164
Yersinia strain that is pathogenic to animals particularly guinea pigs
Y. pseudotuberculosis
165
Delayed citrate reaction
Hafnia
166
Plant pathogen
Erwinia
167
Apron-like colonies in CIN
P. shigelloides
168
Triple decarboxylase positive
P. shigelloides
169
Used to enhance the recovery of Plesiomonas
Inositol Brilliant Green Bile Salt Agar
170
Members of the enterobacteriaciae that grow at 1-5 C
Serratia
| Yersinia
